6DW1
Cryo-EM structure of the benzodiazepine-sensitive alpha1beta1gamma2S tri-heteromeric GABAA receptor in complex with GABA (ECD map)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | D | Gamma-aminobutyric acid receptor subunit gamma-2 | polymer | 490 | 56894.9 | 1 | UniProt (P18508) Pfam (PF02931) Pfam (PF02932) In PDB | Rattus norvegicus (Rat) | GABA(A) receptor subunit gamma-2 |
2 | C, A | Gamma-aminobutyric acid receptor subunit alpha-1,Gamma-aminobutyric acid receptor subunit alpha-1 | polymer | 402 | 46267.1 | 2 | UniProt (P62813) Pfam (PF02931) Pfam (PF02932) In PDB | Rattus norvegicus (Rat) | GABA(A) receptor subunit alpha-1,GABA(A) receptor subunit alpha-1 |
3 | E, B | Gamma-aminobutyric acid receptor subunit beta-1,Gamma-aminobutyric acid receptor subunit beta-1 | polymer | 384 | 44333.0 | 2 | UniProt (P15431) Pfam (PF02931) Pfam (PF02932) In PDB | Rattus norvegicus (Rat) | GABA(A) receptor subunit beta-1,GABA(A) receptor subunit beta-1 |
4 | F | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G81315DD) | |||
5 | G | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G07617FP) | |||
6 | H, J | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
7 | I, K | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
8 | C, A | GAMMA-AMINO-BUTANOIC ACID | non-polymer | 103.1 | 3 | Chemie (ABU) |
Sequence modifications
D: -37 - 428 (UniProt: P18508)
C, A: -26 - 313 (UniProt: P62813)
C, A: 315 - 361 (UniProt: P62813)
E, B: -24 - 308 (UniProt: P15431)
E, B: 311 - 345 (UniProt: P15431)
PDB | External Database | Details |
---|---|---|
Leu 429 | - | expression tag |
Val 430 | - | expression tag |
Pro 431 | - | expression tag |
Arg 432 | - | expression tag |
Gly 433 | - | expression tag |
Ser 434 | - | expression tag |
Arg 435 | - | expression tag |
His 436 | - | expression tag |
His 437 | - | expression tag |
His 438 | - | expression tag |
His 439 | - | expression tag |
His 440 | - | expression tag |
His 441 | - | expression tag |
His 442 | - | expression tag |
His 443 | - | expression tag |
Thr 444 | - | expression tag |
Glu 445 | - | expression tag |
Thr 446 | - | expression tag |
Ser 447 | - | expression tag |
Gln 448 | - | expression tag |
Val 449 | - | expression tag |
Ala 450 | - | expression tag |
Pro 451 | - | expression tag |
Ala 452 | - | expression tag |
C, A: 315 - 361 (UniProt: P62813)
PDB | External Database | Details |
---|---|---|
Thr 314 | - | linker |
Leu 362 | - | expression tag |
Val 363 | - | expression tag |
Pro 364 | - | expression tag |
Arg 365 | - | expression tag |
Gly 366 | - | expression tag |
Ser 367 | - | expression tag |
His 368 | - | expression tag |
His 369 | - | expression tag |
His 370 | - | expression tag |
His 371 | - | expression tag |
His 372 | - | expression tag |
His 373 | - | expression tag |
His 374 | - | expression tag |
His 375 | - | expression tag |
E, B: 311 - 345 (UniProt: P15431)
PDB | External Database | Details |
---|---|---|
Gly 309 | - | linker |
Thr 310 | - | linker |
Leu 346 | - | expression tag |
Val 347 | - | expression tag |
Pro 348 | - | expression tag |
Arg 349 | - | expression tag |
Gly 350 | - | expression tag |
Ser 351 | - | expression tag |
His 352 | - | expression tag |
His 353 | - | expression tag |
His 354 | - | expression tag |
His 355 | - | expression tag |
His 356 | - | expression tag |
His 357 | - | expression tag |
His 358 | - | expression tag |
His 359 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 238095.2 | |
Branched | Number of molecules | 6 |
Total formula weight | 4005.7 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 309.4 | |
All* | Total formula weight | 242410.2 |