6DVE
Crystal structure of Mycobacterium tuberculosis transcription initiation complex(ECF selenomethionine-labelled sigma factor L) with 6 nt spacer
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 359 | 39315.0 | 2 | UniProt (P9WGZ1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1178 | 130018.8 | 1 | UniProt (P9WGY9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1316 | 146969.0 | 1 | UniProt (P9WGY7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E | DNA-directed RNA polymerase subunit omega | polymer | 110 | 11851.1 | 1 | UniProt (P9WGY5) Pfam (PF01192) In PDB | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F | ECF RNA polymerase sigma factor SigL | polymer | 177 | 19703.8 | 1 | UniProt (P9WGH5) Pfam (PF04542) Pfam (PF04545) In PDB | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) | ECF sigma factor SigL,Alternative RNA polymerase sigma factor SigL,RNA polymerase sigma-L factor,Sigma-L factor |
| 6 | G | DNA (5'-D(*GP*CP*AP*TP*CP*CP*GP*TP*GP*AP*GP*T)-3') | polymer | 12 | 3678.4 | 1 | Mycobacterium tuberculosis H37Rv | ||
| 7 | H | DNA (5'-D(P*CP*GP*TP*GP*TP*GP*AP*GP*TP*AP*AP*CP*TP*GP*TP*CP*AP*CP*GP*GP*AP*TP*GP*C)-3') | polymer | 24 | 7385.8 | 1 | Mycobacterium tuberculosis H37Rv | ||
| 8 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
A, B: 1 - 347 (UniProt: P9WGZ1)
| PDB | External Database | Details |
|---|---|---|
| Met -11 | - | initiating methionine |
| Gly -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 398236.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 130.8 | |
| All* | Total formula weight | 398367.7 |
