6DPO

Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 360 s at 21 C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ribonuclease H	polymer	142	16272.4	1	UniProt (Q9KEI9) In PDB	Bacillus halodurans	RNase H
2	B	5'-R(*AP*CP*AP*U)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3'	polymer	4	1224.8	1		synthetic construct
3	b	5'-R(P*CP*G)-3' portion of cleaved RNA (5'-R(*AP*CP*AP*UP*CP*G)-3')	polymer	2	605.4	1		synthetic construct
4	C	DNA (5'-D(*CP*GP*AP*TP*GP*T)-3')	polymer	6	1824.2	1		synthetic construct
5	A, b	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
6	A	IODIDE ION	non-polymer		126.9	4	Chemie (IOD)
7	A, C	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
8	A	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
9	C	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
10		water	water		18.0	171	Chemie (HOH)

Sequence modifications

A: 59 - 196 (UniProt: Q9KEI9)

PDB	External Database	Details
Gly 55	-	expression tag
Ser 56	-	expression tag
His 57	-	expression tag
Met 58	-	expression tag
Ala 188	Glu 188	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	19926.9
Non-Polymers*	Number of molecules	16
	Total formula weight	1266.3
All*	Total formula weight	21193.2

*Water molecules are not included.