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6DPO

Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 360 s at 21 C

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1ARibonuclease Hpolymer14216272.41UniProt (Q9KEI9)
In PDB
Bacillus haloduransRNase H
2B5'-R(*AP*CP*AP*U)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3'polymer41224.81synthetic construct
3b5'-R(P*CP*G)-3' portion of cleaved RNA (5'-R(*AP*CP*AP*UP*CP*G)-3')polymer2605.41synthetic construct
4CDNA (5'-D(*CP*GP*AP*TP*GP*T)-3')polymer61824.21synthetic construct
5A, bMAGNESIUM IONnon-polymer24.33Chemie (MG)
6AIODIDE IONnon-polymer126.94Chemie (IOD)
7A, CGLYCEROLnon-polymer92.15Chemie (GOL)
8A1,2-ETHANEDIOLnon-polymer62.13Chemie (EDO)
9CPOTASSIUM IONnon-polymer39.11Chemie (K)
10waterwater18.0171Chemie (HOH)
Sequence modifications
A: 59 - 196 (UniProt: Q9KEI9)
PDBExternal DatabaseDetails
Gly 55-expression tag
Ser 56-expression tag
His 57-expression tag
Met 58-expression tag
Ala 188Glu 188engineered mutation
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains4
Total formula weight19926.9
Non-Polymers*Number of molecules16
Total formula weight1266.3
All*Total formula weight21193.2
*Water molecules are not included.

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PDB entries from 2024-07-17

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