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6DPG

Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with an RNA/DNA Hybrid: Reaction in 4 mM Mn2+ and 200 mM K+ for 240 s at 21 C

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
Ribonuclease Hpolymer14216272.41UniProt (Q9KEI9)Bacillus haloduransRNase H
2B
(B)
5'-R(*AP*CP*AP*U)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3'polymer41224.81synthetic construct
3C
(b)
5'-R(P*CP*G)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3'polymer2605.41synthetic construct
4D
(C)
DNA (5'-D(*CP*GP*AP*TP*GP*T)-3')polymer61824.21synthetic construct
5E, O, P, R
(A, b)
MANGANESE (II) IONnon-polymer54.94Chemie (MN)
6F, G, H
(A)
IODIDE IONnon-polymer126.93Chemie (IOD)
7I, N
(A)
GLYCEROLnon-polymer92.12Chemie (GOL)
8J, K, L, M
(A)
1,2-ETHANEDIOLnon-polymer62.14Chemie (EDO)
9Q, S
(B, C)
POTASSIUM IONnon-polymer39.12Chemie (K)
10T, U, V, W
(A, B, b, C)
waterwater18.0174Chemie (HOH)
Sequence modifications
A: 59 - 196 (UniProt: Q9KEI9)
PDBExternal DatabaseDetails
Gly 55-expression tag
Ser 56-expression tag
His 57-expression tag
Met 58-expression tag
Ala 188Glu 188engineered mutation
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains4
Total formula weight19926.9
Non-Polymers*Number of molecules15
Total formula weight1111.1
All*Total formula weight21038.0
*Water molecules are not included.

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PDB entries from 2025-08-06

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