6DP4
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 20 mM Mg2+ and 75 mM K+ for 40 s at 21 C
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Ribonuclease H | polymer | 142 | 16330.5 | 1 | UniProt (Q9KEI9) In PDB | Bacillus halodurans | RNase H |
2 | B | 5'-R(*AP*CP*AP*U)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3' | polymer | 4 | 1224.8 | 1 | synthetic construct | ||
3 | b | 5'-R(P*CP*G)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3' | polymer | 2 | 605.4 | 1 | synthetic construct | ||
4 | C | DNA (5'-D(*CP*GP*AP*TP*GP*T)-3') | polymer | 6 | 1824.2 | 1 | synthetic construct | ||
5 | A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
6 | A, C | POTASSIUM ION | non-polymer | 39.1 | 3 | Chemie (K) | |||
7 | A | IODIDE ION | non-polymer | 126.9 | 3 | Chemie (IOD) | |||
8 | A | GLYCEROL | non-polymer | 92.1 | 7 | Chemie (GOL) | |||
9 | water | water | 18.0 | 182 | Chemie (HOH) |
Sequence modifications
A: 59 - 196 (UniProt: Q9KEI9)
PDB | External Database | Details |
---|---|---|
Gly 55 | - | expression tag |
Ser 56 | - | expression tag |
His 57 | - | expression tag |
Met 58 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 19984.9 | |
Non-Polymers* | Number of molecules | 15 |
Total formula weight | 1191.3 | |
All* | Total formula weight | 21176.2 |