6DNH

Cryo-EM structure of human CPSF-160-WDR33-CPSF-30-PAS RNA complex at 3.4 A resolution

Replaces:  6BLL

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cleavage and polyadenylation specificity factor subunit 1	polymer	1443	161074.2	1	UniProt (Q10570) Pfam (PF10433) Pfam (PF03178) In PDB	Homo sapiens (Human)	Cleavage and polyadenylation specificity factor 160 kDa subunit, CPSF 160 kDa subunit
2	B	pre-mRNA 3' end processing protein WDR33	polymer	587	67546.8	1	UniProt (Q9C0J8) Pfam (PF00400) In PDB	Homo sapiens (Human)	WD repeat-containing protein 33, WD repeat-containing protein WDC146
3	C	Cleavage and polyadenylation specificity factor subunit 4	polymer	245	27646.1	1	UniProt (O95639) Pfam (PF00642) Pfam (PF00098) Pfam (PF15663) In PDB	Homo sapiens (Human)	Cleavage and polyadenylation specificity factor 30 kDa subunit, CPSF 30 kDa subunit, NS1 effector domain-binding protein 1, Neb-1, No arches homolog
4	E	RNA (5'-R(P*AP*AP*UP*AP*AP*AP*C)-3')	polymer	17	5361.3	1		Simian virus 40
5	C	ZINC ION	non-polymer		65.4	3	Chemie (ZN)

Sequence modifications

B: 1 - 572 (UniProt: Q9C0J8)

PDB	External Database	Details
Met -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Ser -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Leu -1	-	expression tag
Val 0	-	expression tag

C: 1 - 244 (UniProt: O95639)

PDB	External Database	Details
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	261628.4
Non-Polymers*	Number of molecules	3
	Total formula weight	196.2
All*	Total formula weight	261824.6

*Water molecules are not included.