6DID

HIV Env BG505 SOSIP with polyclonal Fabs from immunized rabbit #3417 post-boost#1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, F, G)	Envelope glycoprotein gp160	polymer	481	54064.3	3	UniProt (Q2N0S6) Pfam (PF00516)	Human immunodeficiency virus 1	Env polyprotein
2	B, D, F (B, I, J)	Envelope glycoprotein gp160	polymer	153	17146.5	3	UniProt (Q2N0S7) Pfam (PF00517)	Human immunodeficiency virus 1	Env polyprotein
3	G, I, K (C, E, H)	Monoclonal antibody 10A light chain	polymer	217	23047.4	3	UniProt (P01840) Pfam (PF07654)	Oryctolagus cuniculus (Rabbit)
4	H, J, L (D, K, L)	Monoclonal antibody 10A heavy chain	polymer	214	22263.0	3	UniProt (A0A1Y1B8B1) Pfam (PF07686) Pfam (PF07654)	Oryctolagus cuniculus (Rabbit)
5	KA, M, Y (k, M, Y)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
6	AA, BA, EA, FA, GA... (a, b, e, f, g...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	21	In PDB GlyTouCan (G42666HT)
7	CA, OA, Q (c, o, Q)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	3	In PDB GlyTouCan (G07617FP)
8	DA, PA, R (d, p, R)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	3	In PDB GlyTouCan (G80966KZ)
9	HA, TA, V (h, t, V)	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	3	In PDB GlyTouCan (G09724ZC)
10	IA, UA, W (i, u, W)	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	3	In PDB GlyTouCan (G22573RC)
11	AB, BB, CB, DB, EB... (A, B, F, I, G...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	21	Chemie (NAG)

Sequence modifications

A, F, G: 31 - 508 (UniProt: Q2N0S6)

PDB	External Database	Details
Asn 332	Thr 330	conflict
Cys 501	Ala 498	conflict
Arg 509	-	expression tag
Arg 510	-	expression tag
Arg 511	-	expression tag
Arg 512	-	expression tag
Arg 513	-	expression tag

B, I, J: 512 - 664 (UniProt: Q2N0S7)

PDB	External Database	Details
Pro 559	Ile 556	conflict
Cys 605	Thr 602	conflict

C, E, H: 21 - 134 (PDB: 6DID)
C, E, H: 135 - 237 (UniProt: P01840)

PDB	External Database	Details
Ser 140	Thr 6	conflict

D, K, L: 21 - 124 (PDB: 6DID)
D, K, L: 125 - 234 (UniProt: A0A1Y1B8B1)

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	349563.6
Branched	Number of molecules	36
	Total formula weight	24034.0
Non-Polymers*	Number of molecules	21
	Total formula weight	4645.4
All*	Total formula weight	378243.0

*Water molecules are not included.