6DE1

Crystal structure of the single mutant (D52N) of the full-length NT5C2 in the active state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cytosolic purine 5'-nucleotidase	polymer	579	66955.7	1	UniProt (P49902) Pfam (PF05761) In PDB	Homo sapiens (Human)	Cytosolic 5'-nucleotidase II
2	A	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
3	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4		water	water		18.0	284	Chemie (HOH)

Sequence modifications

A: 1 - 561 (UniProt: P49902)

PDB	External Database	Details
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 52	Asp 52	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	66955.7
Non-Polymers*	Number of molecules	5
	Total formula weight	472.0
All*	Total formula weight	67427.7

*Water molecules are not included.