6D35
Crystal structure of Xenopus Smoothened in complex with cholesterol
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Smoothened,Soluble cytochrome b562,Smoothened | polymer | 600 | 67568.8 | 1 | UniProt (Q98SW5) UniProt (P0ABE7) Pfam (PF07361) | Xenopus laevis (African clawed frog) | Cytochrome b-562 |
| 2 | B (A) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) |
Sequence modifications
A: 35 - 401 (UniProt: Q98SW5)
A: 1016 - 1120 (UniProt: P0ABE7)
A: 416 - 528 (UniProt: Q98SW5)
| PDB | External Database | Details |
|---|---|---|
| Gly 33 | - | expression tag |
| Pro 34 | - | expression tag |
| Ala 163 | Ser 163 | engineered mutation |
| Val 227 | Ala 227 | conflict |
| Ala 249 | Val 249 | conflict |
| Ser 282 | Asn 282 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Trp 1022 | Met 29 | engineered mutation |
| Ile 1117 | His 124 | engineered mutation |
| Leu 1121 | - | linker |
| Glu 1122 | - | linker |
| Arg 1123 | - | linker |
| Ala 1124 | - | linker |
| Arg 1125 | - | linker |
| Ser 1126 | - | linker |
| Thr 1127 | - | linker |
| Leu 1128 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ala 1011 | - | linker |
| Arg 1012 | - | linker |
| Arg 1013 | - | linker |
| Gln 1014 | - | linker |
| Leu 1015 | - | linker |
| Ala 468 | Thr 468 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 67568.8 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 386.7 | |
| All* | Total formula weight | 67955.4 |
