6D0K

Crystal structure of a CLC-type fluoride/proton antiporter, E118Q mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	CLC-type fluoride/proton antiporter	polymer	421	45667.8	2	UniProt (C9CPP6) In PDB	Enterococcus casseliflavus (strain EC10)
2	D, C	Monobody	polymer	93	9862.8	2		synthetic construct
3	A, B	DECYL-BETA-D-MALTOPYRANOSIDE	non-polymer		482.6	6	Chemie (DMU)
4	A, B	FLUORIDE ION	non-polymer		19.0	4	Chemie (F)
5	C	(CARBAMOYLMETHYL-CARBOXYMETHYL-AMINO)-ACETIC ACID	non-polymer		190.2	1	Chemie (MHA)
6		water	water		18.0	9	Chemie (HOH)

Sequence modifications

A, B: 2 - 406 (UniProt: C9CPP6)

PDB	External Database	Details
Met -2	-	expression tag
Ala -1	-	expression tag
Ala 0	-	expression tag
Ala 1	-	expression tag
Ile 4	Met 4	engineered mutation
Gln 118	Glu 118	engineered mutation
Thr 407	-	expression tag
Arg 408	-	expression tag
Gly 409	-	expression tag
Ser 410	-	expression tag
Gly 411	-	expression tag
Gly 412	-	expression tag
His 413	-	expression tag
His 414	-	expression tag
His 415	-	expression tag
His 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	111061.2
Non-Polymers*	Number of molecules	11
	Total formula weight	3161.5
All*	Total formula weight	114222.7

*Water molecules are not included.