6CUZ

Engineered TrpB from Pyrococcus furiosus, PfTrpB7E6 with (2S,3R)-ethylserine bound as the amino-acrylate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Tryptophan synthase beta chain 1	polymer	388	42361.4	2	UniProt (Q8U093) Pfam (PF00291) In PDB	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
2	B, C	Tryptophan synthase beta chain 1	polymer	388	42589.6	2	UniProt (Q8U093) Pfam (PF00291) In PDB	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
3	A, B, C, D	SODIUM ION	non-polymer		23.0	6	Chemie (NA)
4	A, D	(2E)-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]pent-2-enoic acid	non-polymer		344.3	2	Chemie (FEV)
5	C	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
6		water	water		18.0	550	Chemie (HOH)

Sequence modifications

A, D: 1 - 388 (UniProt: Q8U093)

PDB	External Database	Details
Val 16	Ile 16	engineered mutation
Gly 17	Glu 17	engineered mutation
Pro 91	Leu 91	engineered mutation
Leu 95	Phe 95	engineered mutation
Ala 161	Leu 161	engineered mutation
Glu 173	Val 173	engineered mutation
Leu 274	Phe 274	engineered mutation
Ser 292	Thr 292	engineered mutation
Ala 384	Val 384	engineered mutation

B, C: 1 - 388 (UniProt: Q8U093)

PDB	External Database	Details
Val 16	Ile 16	engineered mutation
Gly 17	Glu 17	engineered mutation
Pro 91	Leu 91	engineered mutation
Leu 95	Phe 95	engineered mutation
Ala 161	Leu 161	engineered mutation
Glu 173	Val 173	engineered mutation
Leu 274	Phe 274	engineered mutation
Ser 292	Thr 292	engineered mutation
Ala 384	Val 384	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	169902.0
Non-Polymers*	Number of molecules	9
	Total formula weight	921.4
All*	Total formula weight	170823.5

*Water molecules are not included.