6CS0

SARS Spike Glycoprotein, Trypsin-cleaved, Stabilized variant, one S1 CTD in an upwards conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Spike glycoprotein,Fibritin	polymer	1215	134739.3	3	UniProt (P59594) UniProt (D9IEJ2) Pfam (PF16451) Pfam (PF09408) Pfam (PF01601) UniProt (by SIFTS) (P10104)	Human SARS coronavirus (SARS-CoV)	S glycoprotein,E2,Peplomer protein
2	D, F, J, K, M... (D, F, J, K, M...)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	9	In PDB GlyTouCan (G15407YE)
3	E (E)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
4	G, H, I, L, O... (G, H, I, L, O...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	8	In PDB GlyTouCan (G42666HT)
5	S (S)	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G22573RC)
6	AA, BA, CA, DA, EA... (B, C, A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	15	Chemie (NAG)

Sequence modifications

A, B, C: 14 - 1190 (UniProt: P59594)

PDB	External Database	Details
Ala 577	Ser 577	conflict
Pro 968	Lys 968	engineered mutation
Pro 969	Val 969	engineered mutation
Gly 1191	-	linker

A, B, C: 1192 - 1219 (UniProt: D9IEJ2)

PDB	External Database	Details
Ser 1192	Ala 457	linker
Gly 1220	-	expression tag
Arg 1221	-	expression tag
Ser 1222	-	expression tag
Leu 1223	-	expression tag
Glu 1224	-	expression tag
Val 1225	-	expression tag
Leu 1226	-	expression tag
Phe 1227	-	expression tag
Gln 1228	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	404217.8
Branched	Number of molecules	19
	Total formula weight	10171.5
Non-Polymers*	Number of molecules	15
	Total formula weight	3318.1
All*	Total formula weight	417707.4

*Water molecules are not included.