6CRX

SARS Spike Glycoprotein, Stabilized variant, two S1 CTDs in the upwards conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, C, A	Spike glycoprotein,Fibritin	polymer	1215	134739.3	3	UniProt (P59594) UniProt (D9IEJ2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) In PDB	Human SARS coronavirus (SARS-CoV)	S glycoprotein,E2,Peplomer protein
2	b, c, D, d, E...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	19	In PDB GlyTouCan (G42666HT)
3	F, J, Z	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
4	a, G, N, O, X	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	5	In PDB GlyTouCan (G81315DD)
5	B, C, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	7	Chemie (NAG)

Sequence modifications

B, C, A: 14 - 1190 (UniProt: P59594)

PDB	External Database	Details
Ala 577	Ser 577	conflict
Pro 968	Lys 968	engineered mutation
Pro 969	Val 969	engineered mutation
Gly 1191	-	linker

B, C, A: 1192 - 1219 (UniProt: D9IEJ2)

PDB	External Database	Details
Ser 1192	Ala 457	linker
Gly 1220	-	expression tag
Arg 1221	-	expression tag
Ser 1222	-	expression tag
Leu 1223	-	expression tag
Glu 1224	-	expression tag
Val 1225	-	expression tag
Leu 1226	-	expression tag
Phe 1227	-	expression tag
Gln 1228	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	404217.8
Branched	Number of molecules	27
	Total formula weight	13566.7
Non-Polymers*	Number of molecules	7
	Total formula weight	1548.5
All*	Total formula weight	419332.9

*Water molecules are not included.