6CA3
THE CRYSTAL STRUCTURE OF THE W169Y MUTANT OF ALPHA-GLUCOSIDASE (GH 31) FROM RUMINOCOCCUS OBEUM ATCC 29174 in complex with miglitol
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Glycosyl hydrolase, family 31 | polymer | 666 | 77426.4 | 2 | UniProt (A5ZY13) Pfam (PF13802) Pfam (PF01055) Pfam (PF21365) | Blautia obeum ATCC 29174 | |
| 2 | C, E (A, B) | (2R,3R,4R,5S)-1-(2-hydroxyethyl)-2-(hydroxymethyl)piperidine-3,4,5-triol | non-polymer | 207.2 | 2 | Chemie (MIG) | |||
| 3 | D (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 4 | F, G (A, B) | water | water | 18.0 | 1084 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 663 (UniProt: A5ZY13)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Tyr 169 | Trp 169 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 154852.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 506.5 | |
| All* | Total formula weight | 155359.4 |
