6CA3
THE CRYSTAL STRUCTURE OF THE W169Y MUTANT OF ALPHA-GLUCOSIDASE (GH 31) FROM RUMINOCOCCUS OBEUM ATCC 29174 in complex with miglitol
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Glycosyl hydrolase, family 31 | polymer | 666 | 77426.4 | 2 | UniProt (A5ZY13) | Blautia obeum ATCC 29174 | |
| 2 | C, E (A, B) | (2R,3R,4R,5S)-1-(2-hydroxyethyl)-2-(hydroxymethyl)piperidine-3,4,5-triol | non-polymer | 207.2 | 2 | Chemie (MIG) PubChem (23622608) PubChem (40467206) PubChem (40467208) PubChem (40477191) PubChem (42052023) PubChem (51577) PubChem (45039933) PubChem (49759862) PubChem (51397099) PubChem (51400355) PubChem (57369304) PubChem (57560749) PubChem (71225356) PubChem (441314) PubChem (69541599) PubChem (69821021) PubChem (71749029) PubChem (89813532) PubChem (124300736) PubChem (93532308) PubChem (6710690) PubChem (9794283) PubChem (124397038) PubChem (124427653) PubChem (124582590) PubChem (12766463) PubChem (134693994) PubChem (134853664) PubChem (145752189) PubChem (158646524) PubChem (168440872) PubChem (171042507) PubChem (173771382) | |||
| 3 | D (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) PubChem (753) | |||
| 4 | F, G (A, B) | water | water | 18.0 | 1084 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 663 (UniProt: A5ZY13)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Tyr 169 | Trp 169 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 154852.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 506.5 | |
| All* | Total formula weight | 155359.4 |
