6C9I

Single-Particle reconstruction of DARP14 - A designed protein scaffold displaying ~17kDa DARPin proteins - Scaffold

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, I...	DARP14 - Subunit A with DARPin	polymer	163	18229.3	12	UniProt (O58404) Pfam (PF01923) In PDB	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
2	E, F, G, H, M...	DARP14 - Subunit B	polymer	131	14346.3	12	UniProt (Q9I2D8) Pfam (PF02962) In PDB	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Sequence modifications

A, B, C, D, I, J, K, L, Q, R, S, T: 1 - 163 (UniProt: O58404)

PDB	External Database	Details
Ala 85	Lys 85	conflict
Leu 88	Glu 88	conflict
Lys 89	Gly 89	conflict
Leu 92	Ser 92	conflict
Met 95	Glu 95	conflict
Leu 126	Glu 126	conflict
Leu 130	Ala 130	conflict
Thr 133	Leu 133	conflict
Ala 140	Lys 140	conflict
Ala 143	Leu 143	conflict
Ala 144	Val 144	conflict
Leu 147	Asn 147	conflict
Ala 148	Arg 148	conflict

E, F, G, H, M, N, O, P, U, V, W, X: 1 - 123 (UniProt: Q9I2D8)

PDB	External Database	Details
Lys 27	Ala 27	conflict
Ile 74	Ala 74	conflict
Thr 78	Gln 78	conflict
Leu 79	Ala 79	conflict
Ala 82	Glu 82	conflict
Ala 86	Glu 86	conflict
Glu 90	Gly 90	conflict
Leu 112	Ala 112	conflict
Leu 124	-	expression tag
Glu 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag
His 131	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	390906.5
All*	Total formula weight	390906.5

*Water molecules are not included.