6C7P

Crystal structure of D477G ACO/RPE65 chimera, monoclinic crystal form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A	Apocarotenoid-15,15'-oxygenase	polymer	490	54401.3	2	UniProt (P74334) Pfam (PF03055) In PDB	Synechocystis sp. (strain PCC 6803 / Kazusa)	ACO,8'-apo-beta-carotenal 15,15'-oxygenase,Diox1
2	B, A	FE (II) ION	non-polymer		55.8	2	Chemie (FE2)
3		water	water		18.0	53	Chemie (HOH)

Sequence modifications

B, A: 1 - 490 (UniProt: P74334)

PDB	External Database	Details
Leu 44	Pro 44	engineered mutation
Trp 45	Asp 45	engineered mutation
Ser 431	Pro 431	engineered mutation
His 432	Arg 432	engineered mutation
Ala 435	Gly 435	engineered mutation
Leu 436	Val 436	engineered mutation
Glu 437	Ala 437	engineered mutation
Val 442	Trp 442	engineered mutation
Asn 461	Asp 461	engineered mutation
Lys 463	Gln 463	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	108802.7
Non-Polymers*	Number of molecules	2
	Total formula weight	111.7
All*	Total formula weight	108914.4

*Water molecules are not included.