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6C6S

CryoEM structure of E.coli RNA polymerase elongation complex bound with RfaH

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1ADNA (29-MER)polymer299001.81Escherichia coli
2BDNA (29-MER)polymer298838.71Escherichia coli
3RRNA (5'-R(*GP*CP*AP*UP*UP*CP*AP*AP*AP*GP*CP*CP*GP*AP*GP*AP*GP*GP*UP*A)-3')polymer206469.91Escherichia coli
4G, HDNA-directed RNA polymerase subunit alphapolymer23926459.12UniProt (P0A7Z4)
Pfam (PF01193)
Pfam (PF01000)
In PDB
Escherichia coli (strain K12)RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
5IDNA-directed RNA polymerase subunit betapolymer1342150820.91UniProt (P0A8V2)
Pfam (PF04560)
Pfam (PF04563)
Pfam (PF04561)
Pfam (PF04565)
Pfam (PF10385)
Pfam (PF00562)
In PDB
Escherichia coli (strain K12)RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
6JDNA-directed RNA polymerase subunit beta'polymer1407155366.81UniProt (P0A8T7)
Pfam (PF04997)
Pfam (PF00623)
Pfam (PF04983)
Pfam (PF05000)
Pfam (PF04998)
In PDB
Escherichia coli (strain K12)RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
7KDNA-directed RNA polymerase subunit omegapolymer9110249.51UniProt (P0A800)
Pfam (PF01192)
In PDB
Escherichia coliRNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
8DTranscription antitermination protein RfaHpolymer16218364.21UniProt (P0AFW0)
Pfam (PF02357)
In PDB
Escherichia coli (strain K12)
9RMAGNESIUM IONnon-polymer24.31Chemie (MG)
10JZINC IONnon-polymer65.42Chemie (ZN)
Sequence modifications
G, H: 1 - 234 (UniProt: P0A7Z4)
PDBExternal DatabaseDetails
Glu 235-expression tag
Val 236-expression tag
Leu 237-expression tag
Phe 238-expression tag
Gln 239-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains9
Total formula weight412030.0
Non-Polymers*Number of molecules3
Total formula weight155.1
All*Total formula weight412185.2
*Water molecules are not included.

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PDB entries from 2024-08-28

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