6C1J
Crystal Structure of Ketosteroid Isomerase Y32F/Y57F/D40N mutant from Pseudomonas Putida (pKSI) bound to 3,4-dinitrophenol
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Steroid Delta-isomerase | polymer | 131 | 14515.5 | 1 | UniProt (P07445) Pfam (PF12680) | Pseudomonas putida (Arthrobacter siderocapsulatus) | Delta(5)-3-ketosteroid isomerase |
| 2 | B, C (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 3 | D (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 4 | E (A) | 3,4-dinitrophenol | non-polymer | 184.1 | 1 | Chemie (DNX) | |||
| 5 | F (A) | water | water | 18.0 | 169 | Chemie (HOH) |
Sequence modifications
A: 1 - 131 (UniProt: P07445)
| PDB | External Database | Details |
|---|---|---|
| Phe 32 | Tyr 32 | engineered mutation |
| Asn 40 | Asp 40 | engineered mutation |
| Phe 57 | Tyr 57 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 14515.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 268.2 | |
| All* | Total formula weight | 14783.7 |
