6BZT

Crystal structure of halogenase PltM L111Y mutant in complex with FAD

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Halogenase PltM	polymer	522	58399.1	4	UniProt (Q4KCZ3)	Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
2	E, L, Q, U (A, B, C, D)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	4	Chemie (FAD)
3	F, M, R, V, W (A, B, C, D)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
4	G, H, I, J, N... (A, B, C, D)	BROMIDE ION	non-polymer		79.9	9	Chemie (BR)
5	K, P (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6	AA, BA, Y, Z (C, D, A, B)	water	water		18.0	943	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 502 (UniProt: Q4KCZ3)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Tyr 111	Leu 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	233596.5
Non-Polymers*	Number of molecules	20
	Total formula weight	4118.8
All*	Total formula weight	237715.3

*Water molecules are not included.