6BYT
Complex structure of LOR107 mutant (R320) with tetrasaccharide substrate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Short ulvan lyase | polymer | 506 | 57517.1 | 2 | UniProt (A0A109PTH9) Pfam (PF15892) In PDB | Alteromonas sp. LOR | |
2 | C, D | 4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid-(1-4)-3-O-sulfo-alpha-L-rhamnopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-4)-3-O-sulfo-alpha-L-rhamnopyranose | branched | 804.7 | 2 | In PDB GlyTouCan (G96014EV) | |||
3 | A, B | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
4 | A, B | SULFATE ION | non-polymer | 96.1 | 7 | Chemie (SO4) | |||
5 | A, B | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
6 | water | water | 18.0 | 325 | Chemie (HOH) |
Sequence modifications
A, B: 26 - 522 (UniProt: A0A109PTH9)
PDB | External Database | Details |
---|---|---|
Met 25 | - | initiating methionine |
Asn 320 | Arg 326 | engineered mutation |
Leu 523 | - | expression tag |
Glu 524 | - | expression tag |
His 525 | - | expression tag |
His 526 | - | expression tag |
His 527 | - | expression tag |
His 528 | - | expression tag |
His 529 | - | expression tag |
His 530 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 115034.2 | |
Branched | Number of molecules | 2 |
Total formula weight | 1609.3 | |
Non-Polymers* | Number of molecules | 14 |
Total formula weight | 1109.0 | |
All* | Total formula weight | 117752.6 |