6BVQ

Crystal structure of 3-hydroxyanthranilate-3,4-dioxygenase N27A from Cupriavidus metallidurans in complex with 4-Cl-3-HAA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	3-hydroxyanthranilate 3,4-dioxygenase	polymer	195	22547.4	1	UniProt (Q1LCS4) Pfam (PF06052)	Cupriavidus metallidurans (Ralstonia metallidurans)	3-hydroxyanthranilate oxygenase,3-HAO,3-hydroxyanthranilic acid dioxygenase,HAD
2	B, C (A)	FE (II) ION	non-polymer		55.8	2	Chemie (FE2)
3	D (A)	4-CHLORO-3-HYDROXYANTHRANILIC ACID	non-polymer		187.6	1	Chemie (4AA)
4	E (A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
5	F (A)	water	water		18.0	96	Chemie (HOH)

Sequence modifications

A: 1 - 174 (UniProt: Q1LCS4)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
His -5	-	expression tag
Ile -4	-	expression tag
Glu -3	-	expression tag
Gly -2	-	expression tag
Arg -1	-	expression tag
His 0	-	expression tag
Ala 27	Asn 27	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	22547.4
Non-Polymers*	Number of molecules	4
	Total formula weight	421.4
All*	Total formula weight	22968.8

*Water molecules are not included.