6BOH

Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, FB (A, FB)	16S ribosomal RNA	polymer	1507	489561.2	2	GenBank (46197919)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
2	B, GB (B, GB)	23S ribosomal RNA	polymer	2880	936785.6	2	GenBank (46197919)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
3	C, HB (C, HB)	5S ribosomal RNA	polymer	120	38882.2	2	GenBank (46197919)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
4	D, IA, IB, NC (D, IA, IB, NC)	tRNA	polymer	77	24846.9	4		Escherichia coli K-12
5	E, JB (E, JB)	50S ribosomal protein L2	polymer	275	30401.4	2	UniProt (Q72I07) Pfam (PF00181) Pfam (PF03947)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
6	F, KB (F, KB)	50S ribosomal protein L3	polymer	206	22450.2	2	UniProt (Q72I04) Pfam (PF00297)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
7	G, LB (G, LB)	50S ribosomal protein L4	polymer	205	22744.3	2	UniProt (Q72I05) Pfam (PF00573)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
8	H, MB (H, MB)	50S ribosomal protein L5	polymer	182	21075.6	2	UniProt (Q72I16) Pfam (PF00281) Pfam (PF00673)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
9	I, NB (I, NB)	50S ribosomal protein L6	polymer	180	19568.9	2	UniProt (Q72I19) Pfam (PF00347)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
10	J, OB (J, OB)	50S ribosomal protein L9	polymer	148	16436.2	2	UniProt (Q72GV5) Pfam (PF01281) Pfam (PF03948)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
11	K, PB (K, PB)	50S ribosomal protein L13	polymer	140	15927.9	2	UniProt (Q72IN1) Pfam (PF00572)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
12	L, QB (L, QB)	50S ribosomal protein L14	polymer	122	13309.6	2	UniProt (Q72I14) Pfam (PF00238)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
13	M, RB (M, RB)	50S ribosomal protein L15	polymer	150	16319.1	2	UniProt (Q72I23) Pfam (PF00828)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
14	N, SB (N, SB)	50S ribosomal protein L16	polymer	141	15977.9	2	UniProt (Q72I11) Pfam (PF00252)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
15	O, TB (O, TB)	50S ribosomal protein L17	polymer	118	13750.1	2	UniProt (Q72I33) Pfam (PF01196)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
16	P, UB (P, UB)	50S ribosomal protein L18	polymer	112	12639.8	2	UniProt (Q72I20) Pfam (PF00861)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
17	Q, VB (Q, VB)	50S ribosomal protein L19	polymer	146	17217.9	2	UniProt (Q72JU9) Pfam (PF01245)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
18	R, WB (R, WB)	50S ribosomal protein L20	polymer	118	13779.3	2	UniProt (Q72L76) Pfam (PF00453)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
19	S, XB (S, XB)	50S ribosomal protein L21	polymer	101	11069.2	2	UniProt (Q72HR2) Pfam (PF00829)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
20	T, YB (T, YB)	50S ribosomal protein L22	polymer	113	12808.1	2	UniProt (Q72I09) Pfam (PF00237)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
21	U, ZB (U, ZB)	50S ribosomal protein L23	polymer	96	10759.8	2	UniProt (Q72I06) Pfam (PF00276)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
22	AC, V (V, AC)	50S ribosomal protein L24	polymer	110	12085.6	2	UniProt (Q72I15) Pfam (PF00467) Pfam (PF17136)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
23	BC, W (W, BC)	50S ribosomal protein L25	polymer	206	23238.6	2	UniProt (Q72IA7) Pfam (PF01386) Pfam (PF14693)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)	General stress protein CTC
24	CC, X (X, CC)	50S ribosomal protein L27	polymer	85	9529.1	2	UniProt (Q72HR3) Pfam (PF01016)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
25	DC, Y (Y, DC)	50S ribosomal protein L28	polymer	98	11032.2	2	UniProt (Q72G84) Pfam (PF00830)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
26	EC, Z (Z, EC)	50S ribosomal protein L29	polymer	72	8670.3	2	UniProt (Q72I12) Pfam (PF00831)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
27	AA, FC (AA, FC)	50S ribosomal protein L30	polymer	60	6799.1	2	UniProt (Q72I22) Pfam (PF00327)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
28	BA, GC (BA, GC)	50S ribosomal protein L31	polymer	71	8300.5	2	UniProt (Q72JR0) Pfam (PF01197)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
29	CA, HC (CA, HC)	50S ribosomal protein L32	polymer	60	6734.1	2	UniProt (P62652) Pfam (PF01783)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
30	DA, IC (DA, IC)	50S ribosomal protein L33	polymer	54	6632.9	2	UniProt (Q72GW3) Pfam (PF00471)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
31	EA, JC (EA, JC)	50S ribosomal protein L34	polymer	49	6132.4	2	UniProt (P80340) Pfam (PF00468)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
32	FA, KC (FA, KC)	50S ribosomal protein L35	polymer	65	7506.2	2	UniProt (Q72L77) Pfam (PF01632)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
33	GA, LC (GA, LC)	50S ribosomal protein L36	polymer	37	4435.4	2	UniProt (Q72I28) Pfam (PF00444)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
34	HA, MC (HA, MC)	mRNA	polymer	27	8862.5	2		synthetic construct
35	JA, KA, OC, PC (JA, KA, OC, PC)	Peptide chain release factor 1	polymer	365	41285.1	4	UniProt (B7MKB3) Pfam (PF03462) Pfam (PF00472) UniProt (by SIFTS) (P0A7I0)	Escherichia coli	RF-1
36	LA, QC (LA, QC)	30S ribosomal protein S2	polymer	256	29317.7	2	UniProt (P62662) Pfam (PF00318)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
37	MA, RC (MA, RC)	30S ribosomal protein S3	polymer	239	26751.1	2	UniProt (P62663) Pfam (PF07650) Pfam (PF00189)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
38	NA, SC (NA, SC)	50S ribosomal protein S4	polymer	209	24373.4	2	UniProt (P62664) Pfam (PF00163) Pfam (PF01479)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
39	OA, TC (OA, TC)	30S ribosomal protein S5	polymer	162	17583.4	2	UniProt (P62665) Pfam (PF00333) Pfam (PF03719)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
40	PA, UC (PA, UC)	30S ribosomal protein S6	polymer	101	11988.8	2	UniProt (P62666) Pfam (PF01250)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
41	QA, VC (QA, VC)	30S ribosomal protein S7	polymer	156	18051.0	2	UniProt (P62667) Pfam (PF00177)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
42	RA, WC (RA, WC)	30S ribosomal protein S8	polymer	138	15868.6	2	UniProt (P62668) Pfam (PF00410)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
43	SA, XC (SA, XC)	30S ribosomal protein S9	polymer	128	14429.7	2	UniProt (P62669) Pfam (PF00380)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
44	TA, YC (TA, YC)	30S ribosomal protein S10	polymer	105	11955.0	2	UniProt (P62653) Pfam (PF00338)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
45	UA, ZC (UA, ZC)	30S ribosomal protein S11	polymer	129	13737.9	2	UniProt (P62654) Pfam (PF00411)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
46	AD, VA (VA, AD)	30S ribosomal protein S12	polymer	132	14683.5	2	UniProt (P61941) Pfam (PF00164)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
47	BD, WA (WA, BD)	30S ribosomal protein S13	polymer	126	14338.9	2	UniProt (P62655) Pfam (PF00416)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
48	CD, XA (XA, CD)	30S ribosomal protein S14 type Z	polymer	61	7158.7	2	UniProt (P62656) Pfam (PF00253)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
49	DD, YA (YA, DD)	30S ribosomal protein S15	polymer	89	10578.4	2	UniProt (P62657) Pfam (PF00312)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
50	ED, ZA (ZA, ED)	30S ribosomal protein S16	polymer	88	10410.0	2	UniProt (P62238) Pfam (PF00886)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
51	AB, FD (AB, FD)	30S ribosomal protein S17	polymer	105	12324.7	2	UniProt (P62658) Pfam (PF00366)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
52	BB, GD (BB, GD)	30S ribosomal protein S18	polymer	88	10258.3	2	UniProt (P62659) Pfam (PF01084)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
53	CB, HD (CB, HD)	30S ribosomal protein S19	polymer	93	10605.5	2	UniProt (P62660) Pfam (PF00203)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
54	DB, ID (DB, ID)	30S ribosomal protein S20	polymer	106	11722.1	2	UniProt (P62661) Pfam (PF01649)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
55	EB, JD (EB, JD)	30S ribosomal protein Thx	polymer	27	3350.0	2	UniProt (P62613) Pfam (PF17070)	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
56	AAA, AAB, ABA, ABB, ACA... (B, GB, D, M, HB...)	MAGNESIUM ION	non-polymer		24.3	1428	Chemie (MG)
57	BNA, LK (GB, B)	BLASTICIDIN S	non-polymer		422.4	2	Chemie (BLS)
58	AFB, EFB, LDA, SEB, TDA... (IC, LC, V, AC, BA...)	ZINC ION	non-polymer		65.4	10	Chemie (ZN)

Sequence modifications

B, GB: 6 - 2897 (GenBank: 46197919)

PDB	External Database	Details
C 154	Unk 1534643	conflict

X, CC: 1 - 85 (UniProt: Q72HR3)

PDB	External Database	Details
Arg 11	Lys 11	conflict

JA, KA, OC, PC: 1 - 357 (UniProt: B7MKB3)

PDB	External Database	Details
-	Asp 302	deletion
-	Arg 303	deletion
-	Ser 304	deletion
Leu 358	-	expression tag
Glu 359	-	expression tag
His 360	-	expression tag
His 361	-	expression tag
His 362	-	expression tag
His 363	-	expression tag
His 364	-	expression tag
His 365	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	114
	Total formula weight	4586347.2
Non-Polymers*	Number of molecules	1440
	Total formula weight	36206.5
All*	Total formula weight	4622553.7

*Water molecules are not included.