6BK8

S. cerevisiae spliceosomal post-catalytic P complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	2	U2 snRNA	polymer	1175	376267.4	1	GenBank (536627)	Saccharomyces cerevisiae
2	5	U5 snRNA	polymer	214	68643.3	1	GenBank (1039024040)	Saccharomyces cerevisiae
3	6	U6 snRNA	polymer	112	35883.2	1	GenBank (1039023280)	Saccharomyces cerevisiae
4	e	RNA (34-MER)	polymer	34	10680.2	1		Saccharomyces cerevisiae
5	i	RNA (59-MER)	polymer	59	18717.9	1		Saccharomyces cerevisiae
6	A	Pre-mRNA-splicing factor Prp8	polymer	2413	279867.5	1	UniProt (P33334) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
7	B	Pre-mRNA-splicing factor SNU114	polymer	1008	114174.0	1	UniProt (P36048) Pfam (PF03764) Pfam (PF00679) Pfam (PF16004) Pfam (PF00009) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10
8	D	Pre-mRNA-splicing factor PRP46	polymer	451	50771.3	1	UniProt (Q12417) Pfam (PF00400) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complexed with CEF1 protein 1,PRP nineteen-associated complex protein 50,PRP19-associated complex protein 50,Pre-mRNA-processing protein 46
9	E	Pre-mRNA-processing protein 45	polymer	379	42548.7	1	UniProt (P28004) Pfam (PF02731) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
10	F	Pre-mRNA-splicing factor SLT11	polymer	364	40988.6	1	UniProt (P38241) Pfam (PF21369) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Extracellular mutant protein 2,Synthetic lethality with U2 protein 11
11	G	Pre-mRNA-splicing factor CWC2	polymer	339	38486.6	1	UniProt (Q12046) Pfam (PF16131) Pfam (PF00076) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complexed with CEF1 protein 2,PRP19-associated complex protein 40,Synthetic lethal with CLF1 protein 3
12	H	Pre-mRNA-splicing factor CWC15	polymer	175	19975.2	1	UniProt (Q03772) Pfam (PF04889) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complexed with CEF1 protein 15
13	I	Pre-mRNA-splicing factor BUD31	polymer	157	18484.5	1	UniProt (P25337) Pfam (PF01125) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Bud site selection protein 31,Complexed with CEF1 protein 14
14	K	Pre-mRNA-splicing factor CWC21	polymer	135	15793.6	1	UniProt (Q03375) Pfam (PF08312) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complexed with CEF1 protein 21
15	L	Pre-mRNA-splicing factor CWC22	polymer	577	67386.1	1	UniProt (P53333) Pfam (PF02847) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Complexed with CEF1 protein 22
16	M	Pre-mRNA-processing factor Prp17	polymer	455	52128.8	1	UniProt (P40968) Pfam (PF00400) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cell division control protein 40
17	N	Pre-mRNA-splicing factor Prp18	polymer	251	28414.4	1	UniProt (P33411) Pfam (PF02840) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
18	O	Pre-mRNA-splicing factor SLU7	polymer	382	44722.9	1	UniProt (Q02775) Pfam (PF11708) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Synthetic lethal with U2 snRNA protein 17,Synthetic lethal with U5 snRNA protein 7
19	P	Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22	polymer	1145	130187.4	1	UniProt (P24384) Pfam (PF00575) Pfam (PF00270) Pfam (PF00271) Pfam (PF04408) Pfam (PF21010) Pfam (PF07717) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
20	R	Pre-mRNA-splicing factor SYF2	polymer	215	24850.7	1	UniProt (P53277) Pfam (PF08231) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	PRP19 complex protein 31,Synthetic lethal with CDC40 protein 2
21	S	Pre-mRNA-splicing factor CEF1	polymer	590	67837.8	1	UniProt (Q03654) Pfam (PF00249) Pfam (PF11831) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	PRP nineteen-associated complex protein 85,PRP19-associated complex protein 85
22	T	Pre-mRNA-splicing factor CLF1	polymer	687	82555.9	1	UniProt (Q12309) Pfam (PF05843) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Crooked neck-like factor 1,PRP19-associated complex protein 77,Synthetic lethal with CDC40 protein 3
23	U	Pre-mRNA-splicing factor SYF1	polymer	859	100344.0	1	UniProt (Q04048) Pfam (PF05843) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	PRP19-associated complex protein 90,Synthetic lethal with CDC40 protein 1
24	X	Unknown protein fragment	polymer	219	18655.9	1		Saccharomyces cerevisiae
25	Y	Unknown protein fragment	polymer	16	1379.7	1		Saccharomyces cerevisiae
26	a, q	Small nuclear ribonucleoprotein Sm D2	polymer	110	12876.1	2	UniProt (Q06217) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Sm-D2,snRNP core protein D2
27	b, m	Small nuclear ribonucleoprotein F	polymer	86	9669.9	2	UniProt (P54999) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	snRNP-F,Sm protein F,SmF
28	c, l	Small nuclear ribonucleoprotein E	polymer	94	10385.1	2	UniProt (Q12330) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	snRNP-E,Sm protein E,SmE
29	d, n	Small nuclear ribonucleoprotein G	polymer	77	8490.8	2	UniProt (P40204) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	snRNP-G,Sm protein G,SmG
30	f, k	Small nuclear ribonucleoprotein-associated protein B	polymer	196	22427.0	2	UniProt (P40018) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	snRNP-B,Sm protein B,SmB
31	g, o	Small nuclear ribonucleoprotein Sm D3	polymer	101	11240.1	2	UniProt (P43321) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Sm-D3,snRNP core protein D3
32	h, p	Small nuclear ribonucleoprotein Sm D1	polymer	146	16296.8	2	UniProt (Q02260) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Sm-D1,snRNP core protein D1
33	r	Lea1	polymer	111	12850.9	1	UniProt (P40567) Pfam (PF13893) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	U2 snRNP B'', U2 small nuclear ribonucleoprotein B''
34	s	Msl1	polymer	238	27232.3	1	UniProt (Q08963) Pfam (PF14580) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	U2 small nuclear ribonucleoprotein A', U2 snRNP A',Looks exceptionally like U2A protein 1
35	u, v, w, x	Pre-mRNA-processing factor Prp19	polymer	503	56629.8	4	UniProt (P32523) Pfam (PF08606) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RING-type E3 ubiquitin transferase PRP19
36	y	Pre-mRNA-splicing factor SNT309	polymer	175	20741.5	1	UniProt (Q06091) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	PRP19-associated complex protein 25,Synergistic to PRP19 mutation protein 309
37	B, 6	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
38	A	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	1	Chemie (IHP)
39	B	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
40	F, G, I, O	ZINC ION	non-polymer		65.4	7	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	46
	Total formula weight	2219860.9
Non-Polymers*	Number of molecules	14
	Total formula weight	1762.6
All*	Total formula weight	2221623.5

*Water molecules are not included.