6BF7

Cryo-EM structure of human insulin degrading enzyme in complex with FAB H11-E heavy chain, FAB H11-E light chain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Insulin-degrading enzyme	polymer	966	111866.5	2	UniProt (P14735) Pfam (PF00675) Pfam (PF05193) Pfam (PF16187)	Homo sapiens (Human)	Abeta-degrading protease,Insulin protease,Insulinase,Insulysin
2	C, E (C, E)	Fab H11-E heavy chain	polymer	218	23057.7	2	UniProt (P0DOX5) Pfam (PF07686) Pfam (PF07654)	Mus musculus (Mouse)
3	D, F (D, F)	Fab H11-E light chain	polymer	211	23087.6	2	UniProt (P0DOX7) Pfam (PF07686) Pfam (PF07654)	Mus musculus (Mouse)

Sequence modifications

A, B: 46 - 1011 (UniProt: P14735)

PDB	External Database	Details
Leu 110	Cys 110	engineered mutation
Ser 171	Cys 171	engineered mutation
Ala 178	Cys 178	engineered mutation
Val 257	Cys 257	engineered mutation
Leu 414	Cys 414	engineered mutation
Asn 573	Cys 573	engineered mutation
Ser 590	Cys 590	engineered mutation
Ser 789	Cys 789	engineered mutation
Ala 812	Cys 812	engineered mutation
Ala 819	Cys 819	engineered mutation
Ser 904	Cys 904	engineered mutation

C, E: 4 - 127 (PDB: 6BF7)
C, E: 127 - 221 (UniProt: P0DOX5)
D, F: 2 - 129 (PDB: 6BF7)
D, F: 130 - 212 (UniProt: P0DOX7)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	316023.7
All*	Total formula weight	316023.7

*Water molecules are not included.