6BD4

Crystal structure of human apo-Frizzled4 receptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Frizzled-4/Rubredoxin chimeric protein	polymer	420	47814.9	1	UniProt (Q9ULV1) UniProt (P00268) Pfam (PF01534) Pfam (PF00301) In PDB	Homo sapiens (Human)
2	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	A	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	4	Chemie (OLC)
4	A	OLEIC ACID	non-polymer		282.5	5	Chemie (OLA)
5	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6	A	UNKNOWN ATOM OR ION	non-polymer			4	Chemie (UNX)
7		water	water		18.0	23	Chemie (HOH)

Sequence modifications

A: 178 - 419 (UniProt: Q9ULV1)

PDB	External Database	Details
Asp 144	-	expression tag
Tyr 145	-	expression tag
Lys 146	-	expression tag
Asp 147	-	expression tag
Asp 148	-	expression tag
Asp 149	-	expression tag
Asp 150	-	expression tag
Ala 151	-	expression tag
Lys 152	-	expression tag
Leu 153	-	expression tag
Gln 154	-	expression tag
Thr 155	-	expression tag
Met 156	-	expression tag
His 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag
His 162	-	expression tag
His 163	-	expression tag
His 164	-	expression tag
His 165	-	expression tag
His 166	-	expression tag
Glu 167	-	expression tag
Asn 168	-	expression tag
Leu 169	-	expression tag
Tyr 170	-	expression tag
Phe 171	-	expression tag
Gln 172	-	expression tag
Gly 173	-	expression tag
Gly 174	-	expression tag
Thr 175	-	expression tag
Leu 176	-	expression tag
Glu 177	-	expression tag
Leu 309	Met 309	engineered mutation

A: 1001 - 1054 (UniProt: P00268)
A: 428 - 517 (UniProt: Q9ULV1)

PDB	External Database	Details
Ile 450	Cys 450	engineered mutation
Phe 507	Cys 507	engineered mutation
Tyr 508	Ser 508	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	47814.9
Non-Polymers*	Number of molecules	17
	Total formula weight	3192.1
All*	Total formula weight	51007.0

*Water molecules are not included.