6BAH

Trastuzumab Fab v3 with 5-diphenyl meditope variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Trastuzumab Fab light chain,Immunoglobulin kappa constant	polymer	214	23518.0	1	UniProt (P01834) Pfam (PF07654) In PDB	Mus musculus	Ig kappa chain C region,Ig kappa chain C region AG,Ig kappa chain C region CUM,Ig kappa chain C region EU,Ig kappa chain C region OU,Ig kappa chain C region ROY,Ig kappa chain C region TI,Ig kappa chain C region,Ig kappa chain C region AG,Ig kappa chain C region CUM,Ig kappa chain C region EU,Ig kappa chain C region OU,Ig kappa chain C region ROY,Ig kappa chain C region TI
2	B	Trastuzumab Fab heavy chain,IGH@ protein	polymer	223	23806.7	1	UniProt (Q6GMX6) Pfam (PF07686) Pfam (PF07654) In PDB	Mus musculus
3	E	Protein L	polymer	64	6907.7	1	UniProt (Q51918) Pfam (PF02246) In PDB	Finegoldia magna (Peptostreptococcus magnus)
4	C	Immunoglobulin G binding protein A	polymer	54	6037.5	1	UniProt (Q2UW42) Pfam (PF02216) UniProt (by SIFTS) (P02976) In PDB	Staphylococcus aureus
5	D	meditope	polymer	13	1591.8	1		synthetic construct
6		water	water		18.0	674	Chemie (HOH)

Sequence modifications

A: 1 - 107 (PDB: 6BAH)
A: 108 - 214 (UniProt: P01834)
B: 1 - 108 (PDB: 6BAH)
B: 109 - 223 (UniProt: Q6GMX6)

PDB	External Database	Details
Cys 175	Ala 190	engineered mutation

E: 21 - 81 (UniProt: Q51918)

PDB	External Database	Details
Gly 18	-	expression tag
Ser 19	-	expression tag
Glu 20	-	expression tag
Ile 34	Thr 490	engineered mutation
Ala 55	Asp 511	engineered mutation
Asn 73	Tyr 529	engineered mutation
His 74	Thr 530	engineered mutation
Met 75	Ile 531	engineered mutation

C: 4 - 54 (UniProt: Q2UW42)

PDB	External Database	Details
Gly 1	-	expression tag
Ser 2	-	expression tag
Tyr 3	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	61861.7
All*	Total formula weight	61861.7

*Water molecules are not included.