6B6H
The cryo-EM structure of a bacterial class I transcription activation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli O157:H7 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (B7MIX3) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) | Escherichia coli O45:K1 (strain S88 / ExPEC) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli O157:H7 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (B7MFL0) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli O45:K1 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase sigma factor RpoD | polymer | 628 | 72206.3 | 1 | UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Escherichia coli (strain K12) | Sigma-70 |
| 6 | G, H (G, H) | cAMP-activated global transcriptional regulator CRP | polymer | 210 | 23672.4 | 2 | UniProt (P0ACK0) Pfam (PF00027) Pfam (PF13545) UniProt (by SIFTS) (P0ACJ8) | Escherichia coli O157:H7 | Catabolite activator protein,CAP,Catabolite gene activator,cAMP receptor protein,CRP,cAMP regulatory protein |
| 7 | I (I) | DNA-directed RNA polymerase subunit alpha | polymer | 75 | 8346.7 | 1 | UniProt (P0A7Z6) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli O157:H7 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 8 | J (1) | SYNTHETIC NONTEMPLATE STRAND DNA (88-MER) | polymer | 88 | 27133.4 | 1 | Escherichia coli | ||
| 9 | K (2) | SYNTHETIC TEMPLATE STRAND DNA (88-MER) | polymer | 88 | 27217.4 | 1 | Escherichia coli | ||
| 10 | L (3) | NASCENT RNA 3-MER | polymer | 3 | 1134.6 | 1 | Escherichia coli | NASCENT RNA 3-MER | |
| 11 | M, N (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 12 | O (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 13 | P, Q (G, H) | ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE | non-polymer | 329.2 | 2 | Chemie (CMP) |
Sequence modifications
F: 1 - 613 (UniProt: P00579)
| PDB | External Database | Details |
|---|---|---|
| Met -14 | - | initiating methionine |
| Arg -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Ser -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| Thr -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Phe -1 | - | expression tag |
| Thr 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 572937.8 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 813.5 | |
| All* | Total formula weight | 573751.4 |
