6B46

Cryo-EM structure of Type I-F CRISPR crRNA-guided Csy surveillance complex with bound anti-CRISPR protein AcrF1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (C, D, E, F, G...)	CRISPR-associated protein Csy3	polymer	344	37781.5	6	UniProt (Q02MM1) Pfam (PF09615)	Pseudomonas aeruginosa (strain UCBPP-PA14)
2	G, H (I, J)	Anti-CRISPR protein AcrF1	polymer	80	8969.1	2	UniProt (L7P7M1) Pfam (PF20829)	Pseudomonas phage JBD30
3	I (L)	CRISPR-associated endonuclease Cas6/Csy4	polymer	189	21629.8	1	UniProt (Q02MM2) Pfam (PF09618)	Pseudomonas aeruginosa (strain UCBPP-PA14)
4	J (M)	Pseudomonas aeruginosa strain SMC4485 CRISPR repeat sequence	polymer	60	19265.4	1	GenBank (313291946)	Pseudomonas aeruginosa

Sequence modifications

C, D, E, F, G, H: 1 - 342 (UniProt: Q02MM1)

PDB	External Database	Details
Met -1	-	initiating methionine
Ala 0	-	expression tag

I, J: 1 - 78 (UniProt: L7P7M1)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

L: 1 - 187 (UniProt: Q02MM2)

PDB	External Database	Details
Met -1	-	initiating methionine
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	285522.6
All*	Total formula weight	285522.6

*Water molecules are not included.