6B44

Cryo-EM structure of Type I-F CRISPR crRNA-guided Csy surveillance complex with bound target dsDNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR-associated protein Csy1	polymer	436	49338.3	1	UniProt (Q02ML9) Pfam (PF09611)	Pseudomonas aeruginosa (strain UCBPP-PA14)
2	B (B)	CRISPR-associated protein Csy2	polymer	329	36446.4	1	UniProt (Q02MM0) Pfam (PF09614)	Pseudomonas aeruginosa (strain UCBPP-PA14)
3	C, D, E, F, G... (C, D, E, F, G...)	CRISPR-associated protein Csy3	polymer	344	37781.5	6	UniProt (Q02MM1) Pfam (PF09615)	Pseudomonas aeruginosa (strain UCBPP-PA14)
4	I (L)	CRISPR-associated endonuclease Cas6/Csy4	polymer	189	21629.8	1	UniProt (Q02MM2) Pfam (PF09618)	Pseudomonas aeruginosa (strain UCBPP-PA14)
5	J (M)	Pseudomonas aeruginosa strain SMC4485 CRISPR repeat sequence	polymer	60	19265.4	1	GenBank (313291946)	Pseudomonas aeruginosa
6	K (N)	Target DNA strand (41-MER)	polymer	49	15191.7	1		synthetic construct
7	L (O)	Non-target DNA strand (5'-D(P*CP*AP*GP*TP*CP*AP*TP*CP*AP*CP*CP*AP*A)-3')	polymer	27	8216.3	1		synthetic construct

Sequence modifications

A: 1 - 434 (UniProt: Q02ML9)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

B: 1 - 327 (UniProt: Q02MM0)

PDB	External Database	Details
Met -1	-	initiating methionine
Ala 0	-	expression tag

C, D, E, F, G, H: 1 - 342 (UniProt: Q02MM1)

PDB	External Database	Details
Met -1	-	initiating methionine
Ala 0	-	expression tag

L: 1 - 187 (UniProt: Q02MM2)

PDB	External Database	Details
Met -1	-	initiating methionine
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	376777.2
All*	Total formula weight	376777.2

*Water molecules are not included.