6AZS
Structural and biochemical characterization of a non-canonical biuret hydrolase (BiuH) from the cyanuric acid catabolism pathway of Rhizobium leguminasorum bv. viciae 3841
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Putative amidase | polymer | 262 | 28592.6 | 4 | UniProt (Q1M7F4) Pfam (PF00857) In PDB | Rhizobium leguminosarum bv. viciae (strain 3841) | |
2 | A, B, C, D | 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 209.2 | 4 | Chemie (BTB) | |||
3 | A, C, D | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
4 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
5 | B, D | N-formyl-D-aspartic acid | non-polymer | 161.1 | 2 | Chemie (C5S) | |||
6 | D | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
7 | water | water | 18.0 | 888 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 233 (UniProt: Q1M7F4)
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Ala 142 | Lys 142 | conflict |
Gly 234 | - | expression tag |
Leu 235 | - | expression tag |
Val 236 | - | expression tag |
Pro 237 | - | expression tag |
Arg 238 | - | expression tag |
Gly 239 | - | expression tag |
Ser 240 | - | expression tag |
Ile 241 | - | expression tag |
Glu 242 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 114370.3 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 1577.0 | |
All* | Total formula weight | 115947.3 |