6AZO

Structural and biochemical characterization of a non-canonical biuret hydrolase (BiuH) from the cyanuric acid catabolism pathway of Rhizobium leguminasorum bv. viciae 3841

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Putative amidase	polymer	262	29072.7	4	UniProt (Q1M7F4) Pfam (PF00857) In PDB	Rhizobium leguminosarum bv. viciae (strain 3841)
2	C, D	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
3		water	water		18.0	413	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 233 (UniProt: Q1M7F4)

PDB	External Database	Details
Mse -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gly 234	-	expression tag
Leu 235	-	expression tag
Val 236	-	expression tag
Pro 237	-	expression tag
Arg 238	-	expression tag
Gly 239	-	expression tag
Ser 240	-	expression tag
Ile 241	-	expression tag
Glu 242	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	116290.9
Non-Polymers*	Number of molecules	2
	Total formula weight	70.9
All*	Total formula weight	116361.8

*Water molecules are not included.