6AYU

Crystal structure of fructose-1,6-bisphosphatase T84S from Mycobacterium tuberculosis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Fructose-1,6-bisphosphatase class 2	polymer	347	36646.4	2	UniProt (A5U1E6) Pfam (PF03320) UniProt (by SIFTS) (P9WN21)	Mycobacterium tuberculosis	FBPase class 2,D-fructose-1,6-bisphosphate 1-phosphohydrolase class 2
2	C, J (A, B)	6-O-phosphono-beta-D-fructofuranose	non-polymer		260.1	2	Chemie (F6P)
3	D, H, L (A, B)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
4	E (A)	MALONATE ION	non-polymer		102.0	1	Chemie (MLI)
5	F, G, I, K, M... (A, B)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
6	P, Q (A, B)	water	water		18.0	164	Chemie (HOH)

Sequence modifications

A, B: 1 - 328 (UniProt: A5U1E6)

PDB	External Database	Details
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 84	Thr 118	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73292.8
Non-Polymers*	Number of molecules	13
	Total formula weight	1339.9
All*	Total formula weight	74632.7

*Water molecules are not included.