6AX9

F95N Epi-isozizaene synthase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Epi-isozizaene synthase	polymer	382	43682.9	1	UniProt (Q9K499) Pfam (PF19086)	Streptomyces coelicolor	EIZS,Sesquiterpene cyclase,Sesquiterpene synthase
2	B, C, D (A)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
3	E (A)	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)
4	F (A)	N-benzyl-N,N-diethylethanaminium	non-polymer		192.3	1	Chemie (BTM)
5	G (A)	water	water		18.0	48	Chemie (HOH)

Sequence modifications

A: 2 - 361 (UniProt: Q9K499)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Val 1	-	expression tag
Asn 95	Phe 95	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	43682.9
Non-Polymers*	Number of molecules	5
	Total formula weight	441.2
All*	Total formula weight	44124.1

*Water molecules are not included.