6ASX
CryoEM structure of E.coli his pause elongation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | DNA (32-MER) | polymer | 32 | 9881.4 | 1 | Salmonella enterica subsp. enterica serovar Typhimurium | Non-template DNA | |
| 2 | B | DNA (32-MER) | polymer | 32 | 9706.3 | 1 | Salmonella enterica subsp. enterica serovar Typhimurium | template DNA | |
| 3 | R | RNA (29-MER) | polymer | 29 | 9231.4 | 1 | Salmonella enterica subsp. enterica serovar Typhimurium | ||
| 4 | G, H | DNA-directed RNA polymerase subunit alpha | polymer | 239 | 26459.1 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) In PDB | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 5 | I | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB | Escherichia coli (strain K12) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 6 | J | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli (strain K12) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 7 | K | DNA-directed RNA polymerase subunit omega | polymer | 84 | 9493.7 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 8 | R | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 9 | J | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
G, H: 1 - 234 (UniProt: P0A7Z4)
| PDB | External Database | Details |
|---|---|---|
| Glu 235 | - | expression tag |
| Val 236 | - | expression tag |
| Leu 237 | - | expression tag |
| Phe 238 | - | expression tag |
| Gln 239 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 397418.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 397573.8 |
