6AOA

Monomeric crystal structure of the E497/C566D double mutant of the guanylyl cyclase domain of the RhoGC fusion protein from the aquatic fungus Blastocladiella emersonii

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Bacterio-rhodopsin/guanylyl cyclase 1 fusion protein	polymer	192	21514.6	1	UniProt (A0A060H1D7) Pfam (PF00211)	Blastocladiella emersonii (Aquatic fungus)
2	B (A)	water	water		18.0	320	Chemie (HOH)

Sequence modifications

A: 443 - 626 (UniProt: A0A060H1D7)

PDB	External Database	Details
Met 442	-	initiating methionine
Lys 497	Glu 497	engineered mutation
Asp 566	Cys 566	engineered mutation
Met 627	-	cloning artifact
His 628	-	expression tag
His 629	-	expression tag
His 630	-	expression tag
His 631	-	expression tag
His 632	-	expression tag
His 633	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	21514.6
All*	Total formula weight	21514.6

*Water molecules are not included.