6AM8

Engineered tryptophan synthase b-subunit from Pyrococcus furiosus, PfTrpB2B9 with Trp bound as E(Aex2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Tryptophan synthase beta chain 1	polymer	396	43618.7	2	UniProt (Q8U093) Pfam (PF00291)	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
2	B, C (B, C)	Tryptophan synthase beta chain 1	polymer	396	43390.6	2	UniProt (Q8U093) Pfam (PF00291)	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
3	E, G, L (A, B, D)	TRYPTOPHAN	non-polymer		204.2	3	Chemie (TRP)
4	F, I, K, M (A, B, C, D)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
5	H, J (B, C)	[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-L-TRYPTOPHANE	non-polymer		433.4	2	Chemie (PLT)
6	N, O, P, Q (A, B, C, D)	water	water		18.0	407	Chemie (HOH)

Sequence modifications

A, D: 1 - 388 (UniProt: Q8U093)

PDB	External Database	Details
Val 16	Ile 16	engineered mutation
Gly 17	Glu 17	engineered mutation
Val 68	Ile 68	engineered mutation
Leu 95	Phe 95	engineered mutation
Ser 274	Phe 274	engineered mutation
Ser 292	Thr 292	engineered mutation
Ala 321	Thr 321	engineered mutation
Ala 384	Val 384	engineered mutation
Leu 389	-	expression tag
Glu 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag

B, C: 1 - 388 (UniProt: Q8U093)

PDB	External Database	Details
Val 16	Ile 16	engineered mutation
Gly 17	Glu 17	engineered mutation
Val 68	Ile 68	engineered mutation
Leu 95	Phe 95	engineered mutation
Ser 274	Phe 274	engineered mutation
Ser 292	Thr 292	engineered mutation
Ala 321	Thr 321	engineered mutation
Ala 384	Val 384	engineered mutation
Leu 389	-	expression tag
Glu 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	174018.6
Non-Polymers*	Number of molecules	9
	Total formula weight	1571.3
All*	Total formula weight	175590.0

*Water molecules are not included.