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6ALG

CryoEM structure of HK022 Nun - E.coli RNA polymerase elongation complex

Replaces:  5UP6
Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
DNA (29-MER)polymer298840.71Enterobacteria phage T7
2B
(B)
DNA (29-MER)polymer298813.61Enterobacteria phage T7
3C
(R)
RNA (5'-R(P*CP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3')polymer206510.01Enterobacteria phage T7
4D, E
(G, H)
DNA-directed RNA polymerase subunit alphapolymer23926459.12UniProt (P0A7Z4)
Pfam (PF01193)
Pfam (PF01000)
Escherichia coli (strain K12)RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
5F
(I)
DNA-directed RNA polymerase subunit betapolymer1342150820.91UniProt (P0A8V2)
Pfam (PF04563)
Pfam (PF04561)
Pfam (PF04565)
Pfam (PF10385)
Pfam (PF00562)
Pfam (PF04560)
Escherichia coli (strain K12)RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
6G
(J)
DNA-directed RNA polymerase subunit beta'polymer1407155366.81UniProt (P0A8T7)
Pfam (PF04997)
Pfam (PF00623)
Pfam (PF04983)
Pfam (PF05000)
Pfam (PF04998)
Escherichia coli (strain K12)RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
7H
(K)
DNA-directed RNA polymerase subunit omegapolymer9110249.51UniProt (P0A800)
Pfam (PF01192)
Escherichia coli (strain K12)RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
8I
(N)
Transcription termination factor nunpolymer10912758.41UniProt (P18683)Escherichia phage HK022
9J
(J)
MAGNESIUM IONnon-polymer24.31Chemie (MG)
10K, L
(J)
ZINC IONnon-polymer65.42Chemie (ZN)
Sequence modifications
G, H: 1 - 234 (UniProt: P0A7Z4)
PDBExternal DatabaseDetails
Glu 235-expression tag
Val 236-expression tag
Leu 237-expression tag
Phe 238-expression tag
Gln 239-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains9
Total formula weight406278.1
Non-Polymers*Number of molecules3
Total formula weight155.1
All*Total formula weight406433.3
*Water molecules are not included.

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PDB entries from 2025-06-11

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