6AIF

Crystal structure of M120A mutant of O-acetylserine sulfhydrylase from Haemophilus influenzae in complex with high affinity inhibitory peptide from serine acetyl transferase of Salmonella typhimurium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cysteine synthase	polymer	350	37144.3	1	UniProt (P45040) Pfam (PF00291) In PDB	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	CSase,O-acetylserine (thiol)-lyase,OAS-TL,O-acetylserine sulfhydrylase
2	B	Peptide from Serine acetyltransferase	polymer	10	1225.3	1	UniProt (P29847) In PDB	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	SAT,Serine transacetylase
3		water	water		18.0	46	Chemie (HOH)

Sequence modifications

A: 1 - 316 (UniProt: P45040)

PDB	External Database	Details
Met -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 120	Met 120	engineered mutation

B: 1 - 10 (UniProt: P29847)

PDB	External Database	Details
Trp 1	His 264	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	38369.6
All*	Total formula weight	38369.6

*Water molecules are not included.