6AGS

Structural insights for non-natural cofactor binding by the L310R/Q401C mutant of malic enzyme from Escherichia coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	NAD-dependent malic enzyme	polymer	573	64348.2	1	UniProt (A0A1X7B3B3) Pfam (PF00390) Pfam (PF03949) UniProt (by SIFTS) (P26616)	Escherichia coli	NAD-ME
2	B (A)	water	water		18.0	200	Chemie (HOH)

Sequence modifications

A: 1 - 565 (UniProt: A0A1X7B3B3)

PDB	External Database	Details
Arg 301	Leu 310	engineered mutation
Cys 392	Gln 401	engineered mutation
Leu 566	-	expression tag
Glu 567	-	expression tag
His 568	-	expression tag
His 569	-	expression tag
His 570	-	expression tag
His 571	-	expression tag
His 572	-	expression tag
His 573	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	64348.2
All*	Total formula weight	64348.2

*Water molecules are not included.