6A0H

Crystal structure of human protein N-terminal asparagine amidohydrolase (NTAN1) C75S mutant with Asn-Leu-Ala-Ala-Arg peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Protein N-terminal asparagine amidohydrolase	polymer	318	35778.2	2	UniProt (Q96AB6) Pfam (PF14736)	Homo sapiens (Human)	Protein NH2-terminal asparagine amidohydrolase,PNAA,Protein NH2-terminal asparagine deamidase,Protein NTN-amidase
2	C, D (C, D)	5-mer peptide ASN-LEU-ALA-ALA-ARG	polymer	5	544.6	2		synthetic construct
3	E, F, G, K, L (A, B)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
4	H, I, J, M, N... (A, B)	PHOSPHATE ION	non-polymer		95.0	6	Chemie (PO4)
5	P, Q (A, B)	water	water		18.0	13	Chemie (HOH)

Sequence modifications

A, B: 1 - 310 (UniProt: Q96AB6)

PDB	External Database	Details
Ser 75	Cys 75	engineered mutation
Leu 311	-	expression tag
Glu 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	72645.7
Non-Polymers*	Number of molecules	11
	Total formula weight	1030.3
All*	Total formula weight	73676.0

*Water molecules are not included.