5ZX3

Mycobacterium tuberculosis RNA polymerase holoenzyme with ECF sigma factor sigma H

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	368	40279.0	2	UniProt (P9WGZ1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Mycobacterium tuberculosis H37Rv	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1174	129602.3	1	UniProt (P9WGY9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560)	Mycobacterium tuberculosis H37Rv	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1317	147068.1	1	UniProt (P9WGY7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Mycobacterium tuberculosis H37Rv	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	110	11851.1	1	UniProt (P9WGY5) Pfam (PF01192)	Mycobacterium tuberculosis H37Rv	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (F)	ECF RNA polymerase sigma factor SigH	polymer	218	24382.2	1	UniProt (P9WGH9) Pfam (PF04542) Pfam (PF08281)	Mycobacterium tuberculosis H37Rv	ECF sigma factor SigH,Alternative RNA polymerase sigma factor SigH,RNA polymerase sigma-H factor,Sigma-H factor
6	G, H (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	I, J, K, L (A, C, D, F)	water	water		18.0	53	Chemie (HOH)

Sequence modifications

A, B: 1 - 347 (UniProt: P9WGZ1)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
His -5	-	expression tag
Ile -4	-	expression tag
Glu -3	-	expression tag
Gly -2	-	expression tag
Arg -1	-	expression tag
His 0	-	expression tag

C: 7 - 1178 (UniProt: P9WGY9)

PDB	External Database	Details
Met 5	-	expression tag
Val 6	-	expression tag

D: 2 - 1316 (UniProt: P9WGY7)

PDB	External Database	Details
Met 0	-	expression tag
Val 1	-	expression tag

F: 1 - 216 (UniProt: P9WGH9)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	393461.8
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	393592.7

*Water molecules are not included.