5ZWN
Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.3 angstrom (Part II: U1 snRNP region)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | G | pre-mRNA | polymer | 22 | 7130.4 | 1 | Saccharomyces cerevisiae S288c | ||
| 2 | P | U1 snRNA | polymer | 568 | 182114.5 | 1 | Saccharomyces cerevisiae S288c | ||
| 3 | Q | U1 small nuclear ribonucleoprotein 70 kDa homolog | polymer | 300 | 34506.1 | 1 | UniProt (Q00916) Pfam (PF12220) Pfam (PF00076) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | U1-70K,U1 small nuclear ribonucleoprotein SNP1,U1 snRNP protein SNP1 |
| 4 | R | U1 small nuclear ribonucleoprotein C | polymer | 231 | 27106.0 | 1 | UniProt (Q05900) Pfam (PF06220) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | U1C |
| 5 | S | U1 small nuclear ribonucleoprotein A | polymer | 298 | 34438.1 | 1 | UniProt (P32605) Pfam (PF00076) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | U1A,Mutant U1 die protein 1 |
| 6 | T | U1 small nuclear ribonucleoprotein component PRP42 | polymer | 544 | 65222.0 | 1 | UniProt (Q03776) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | U1 snRNP protein PRP42,65 kDa snRNP protein,Pre-mRNA-processing factor 42 |
| 7 | U | Pre-mRNA-processing factor 39 | polymer | 629 | 74834.7 | 1 | UniProt (P39682) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 8 | V | Protein NAM8 | polymer | 523 | 57010.8 | 1 | UniProt (Q00539) Pfam (PF00076) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 9 | W | 56 kDa U1 small nuclear ribonucleoprotein component | polymer | 492 | 56575.3 | 1 | UniProt (Q03782) Pfam (PF19097) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 10 | a | Small nuclear ribonucleoprotein-associated protein B | polymer | 196 | 22427.0 | 1 | UniProt (P40018) Pfam (PF01423) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-B,Sm protein B,SmB |
| 11 | b | Small nuclear ribonucleoprotein Sm D1 | polymer | 146 | 16296.8 | 1 | UniProt (Q02260) Pfam (PF01423) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | Sm-D1,snRNP core protein D1 |
| 12 | c | Small nuclear ribonucleoprotein Sm D2 | polymer | 110 | 12876.1 | 1 | UniProt (Q06217) Pfam (PF01423) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | Sm-D2,snRNP core protein D2 |
| 13 | d | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 1 | UniProt (P43321) Pfam (PF01423) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | Sm-D3,snRNP core protein D3 |
| 14 | e | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 1 | UniProt (Q12330) Pfam (PF01423) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-E,Sm protein E,SmE |
| 15 | f | Small nuclear ribonucleoprotein F | polymer | 86 | 9669.9 | 1 | UniProt (P54999) Pfam (PF01423) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-F,Sm protein F,SmF |
| 16 | g | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 1 | UniProt (P40204) Pfam (PF01423) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-G,Sm protein G,SmG |
| 17 | X | U1 small nuclear ribonucleoprotein component SNU71 | polymer | 619 | 71383.5 | 1 | UniProt (P53207) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 18 | Y | Protein LUC7 | polymer | 261 | 30245.9 | 1 | UniProt (Q07508) Pfam (PF03194) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 19 | x | U1 snRNP | polymer | 49 | 3500.8 | 1 | Saccharomyces cerevisiae S288c | ||
| 20 | y | Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 | polymer | 588 | 66733.2 | 1 | UniProt (P23394) Pfam (PF00270) Pfam (PF00271) In PDB | Saccharomyces cerevisiae S288c (Baker's yeast) | Helicase CA8 |
| 21 | R, Y | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 20 |
| Total formula weight | 802187.2 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 196.2 | |
| All* | Total formula weight | 802383.5 |
