5ZWM
Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part)
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pre-mRNA-splicing factor 8 | polymer | 2413 | 279867.5 | 1 | UniProt (P33334) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 2 | B (K) | U4/U6 small nuclear ribonucleoprotein PRP4 | polymer | 465 | 52507.0 | 1 | UniProt (P20053) Pfam (PF00400) | Saccharomyces cerevisiae S288c (Baker's yeast) | Pre-mRNA-processing protein 4 |
| 3 | C (L) | Pre-mRNA-processing factor 31 | polymer | 494 | 56382.5 | 1 | UniProt (P49704) Pfam (PF01798) Pfam (PF09785) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 4 | D (N) | Pre-mRNA-splicing factor 6 | polymer | 899 | 104370.1 | 1 | UniProt (P19735) Pfam (PF06424) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 5 | E (J) | U4/U6 small nuclear ribonucleoprotein PRP3 | polymer | 469 | 55974.3 | 1 | UniProt (Q03338) Pfam (PF08572) Pfam (PF06544) | Saccharomyces cerevisiae S288c (Baker's yeast) | Pre-mRNA-splicing factor 3 |
| 6 | F (E) | Spliceosomal protein DIB1 | polymer | 143 | 16798.4 | 1 | UniProt (Q06819) Pfam (PF02966) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 7 | G (M) | 13 kDa ribonucleoprotein-associated protein | polymer | 126 | 13582.9 | 1 | UniProt (P39990) Pfam (PF01248) | Saccharomyces cerevisiae S288c (Baker's yeast) | Small nuclear ribonucleoprotein-associated protein 1 |
| 8 | H (C) | Pre-mRNA-splicing factor SNU114 | polymer | 1008 | 114174.0 | 1 | UniProt (P36048) Pfam (PF16004) Pfam (PF00009) Pfam (PF03764) Pfam (PF00679) | Saccharomyces cerevisiae S288c (Baker's yeast) | 114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10 |
| 9 | I (z) | U6 snRNA-associated Sm-like protein LSm8 | polymer | 109 | 12403.4 | 1 | UniProt (P47093) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 10 | J (q) | U6 snRNA-associated Sm-like protein LSm2 | polymer | 95 | 11177.9 | 1 | UniProt (P38203) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | Small nuclear ribonucleoprotein D homolog SNP3 |
| 11 | K (r) | U6 snRNA-associated Sm-like protein LSm3 | polymer | 89 | 10039.3 | 1 | UniProt (P57743) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | SmX4 protein |
| 12 | L (x) | U6 snRNA-associated Sm-like protein LSm6 | polymer | 86 | 9406.6 | 1 | UniProt (Q06406) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 13 | M (t) | U6 snRNA-associated Sm-like protein LSm5 | polymer | 93 | 10433.0 | 1 | UniProt (P40089) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 14 | N (y) | U6 snRNA-associated Sm-like protein LSm7 | polymer | 115 | 13027.0 | 1 | UniProt (P53905) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 15 | O (s) | U6 snRNA-associated Sm-like protein LSm4 | polymer | 187 | 21298.1 | 1 | UniProt (P40070) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 16 | P (F) | U6 snRNA | polymer | 112 | 35846.1 | 1 | Saccharomyces cerevisiae S288c | ||
| 17 | Q (I) | U4 snRNA | polymer | 160 | 51186.0 | 1 | Saccharomyces cerevisiae S288c | ||
| 18 | R (B) | U5 snRNA | polymer | 214 | 68643.3 | 1 | Saccharomyces cerevisiae S288c | ||
| 19 | S (O) | 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component | polymer | 587 | 66544.0 | 1 | UniProt (Q12420) Pfam (PF19252) Pfam (PF03343) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 20 | EA, OA, T (d, S, l) | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 3 | UniProt (P43321) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | Sm-D3,snRNP core protein D3 |
| 21 | BA, KA, U (a, P, h) | Small nuclear ribonucleoprotein-associated protein B | polymer | 196 | 22427.0 | 3 | UniProt (P40018) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-B,Sm protein B,SmB |
| 22 | CA, PA, V (b, Q, m) | Small nuclear ribonucleoprotein Sm D1 | polymer | 146 | 16296.8 | 3 | UniProt (Q02260) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | Sm-D1,snRNP core protein D1 |
| 23 | DA, QA, W (c, R, n) | Small nuclear ribonucleoprotein Sm D2 | polymer | 110 | 12876.1 | 3 | UniProt (Q06217) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | Sm-D2,snRNP core protein D2 |
| 24 | FA, LA, X (e, T, i) | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 3 | UniProt (Q12330) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-E,Sm protein E,SmE |
| 25 | GA, MA, Y (f, U, j) | Small nuclear ribonucleoprotein F | polymer | 86 | 9669.9 | 3 | UniProt (P54999) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-F,Sm protein F,SmF |
| 26 | HA, NA, Z (g, V, k) | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 3 | UniProt (P40204) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-G,Sm protein G,SmG |
| 27 | AA (D) | Pre-mRNA-splicing helicase BRR2 | polymer | 2163 | 246470.3 | 1 | UniProt (P32639) Pfam (PF21188) Pfam (PF18149) Pfam (PF00270) Pfam (PF00271) Pfam (PF23445) Pfam (PF02889) | Saccharomyces cerevisiae S288c (Baker's yeast) | Protein Snu246 |
| 28 | IA (G) | Pre-mRNA-BPS | polymer | 44 | 13984.3 | 1 | Saccharomyces cerevisiae S288c | ||
| 29 | JA (H) | U2 snRNA | polymer | 1175 | 376267.4 | 1 | Saccharomyces cerevisiae S288c | ||
| 30 | RA (o) | U2 small nuclear ribonucleoprotein A' | polymer | 238 | 27232.3 | 1 | UniProt (Q08963) Pfam (PF14580) | Saccharomyces cerevisiae S288c (Baker's yeast) | U2 snRNP A',Looks exceptionally like U2A protein 1 |
| 31 | SA (p) | U2 small nuclear ribonucleoprotein B'' | polymer | 111 | 12850.9 | 1 | UniProt (P40567) Pfam (PF13893) | Saccharomyces cerevisiae S288c (Baker's yeast) | U2 snRNP B'' |
| 32 | TA (1) | U2 snRNP component HSH155 | polymer | 971 | 110166.7 | 1 | UniProt (P49955) Pfam (PF22646) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 33 | UA (2) | Cold sensitive U2 snRNA suppressor 1 | polymer | 436 | 50339.9 | 1 | UniProt (Q02554) Pfam (PF04037) Pfam (PF04046) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 34 | VA (3) | Pre-mRNA-splicing factor RSE1 | polymer | 1361 | 153956.8 | 1 | UniProt (Q04693) Pfam (PF10433) Pfam (PF23726) Pfam (PF03178) | Saccharomyces cerevisiae S288c (Baker's yeast) | RNA splicing and ER to Golgi transport factor 1,Spliceosome-associated protein 130 |
| 35 | WA (4) | Protein HSH49 | polymer | 213 | 24534.2 | 1 | UniProt (Q99181) Pfam (PF00076) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 36 | XA (5) | Pre-mRNA-splicing factor RDS3 | polymer | 107 | 12283.6 | 1 | UniProt (Q06835) Pfam (PF03660) | Saccharomyces cerevisiae S288c (Baker's yeast) | Regulator of drug sensitivity 3 |
| 37 | YA (6) | RDS3 complex subunit 10 | polymer | 85 | 10045.4 | 1 | UniProt (P0C074) Pfam (PF07189) | Saccharomyces cerevisiae S288c (Baker's yeast) | Splicing factor 3b subunit |
| 38 | ZA (X) | U2 snRNP component IST3 | polymer | 148 | 17121.1 | 1 | UniProt (P40565) Pfam (PF00076) | Saccharomyces cerevisiae S288c (Baker's yeast) | Increased sodium tolerance protein 3,U2 snRNP protein SNU17 |
| 39 | AB (Y) | Pre-mRNA-splicing factor CWC26 | polymer | 266 | 30529.1 | 1 | UniProt (P46947) Pfam (PF09736) | Saccharomyces cerevisiae S288c (Baker's yeast) | Bud site selection protein 13,Complexed with CEF1 protein 26,Synthetic lethal with CLF1 protein 7 |
| 40 | BB (Z) | Pre-mRNA leakage protein 1 | polymer | 204 | 23685.7 | 1 | UniProt (Q07930) Pfam (PF00498) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 41 | CB (u) | Pre-mRNA-splicing factor PRP9 | polymer | 530 | 63126.4 | 1 | UniProt (P19736) Pfam (PF16958) Pfam (PF16837) Pfam (PF12171) Pfam (PF11931) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 42 | DB (w) | Pre-mRNA-splicing factor PRP21 | polymer | 280 | 33111.5 | 1 | UniProt (P32524) Pfam (PF01805) Pfam (PF12230) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 43 | EB (v) | Pre-mRNA-splicing factor PRP11 | polymer | 266 | 29962.8 | 1 | UniProt (Q07350) Pfam (PF12874) Pfam (PF16835) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 44 | FB (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 45 | GB (C) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 46 | HB, IB, JB, KB, LB... (5, u, v) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 57 |
| Total formula weight | 2513487.3 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 939.9 | |
| All* | Total formula weight | 2514427.2 |
