5ZTY

Crystal structure of human G protein coupled receptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	G protein coupled receptor,T4 lysozyme,G protein coupled receptor	polymer	500	56195.9	1	UniProt (P34972) UniProt (D9IEF7) Pfam (PF00001) Pfam (PF00959) In PDB	Homo sapiens (Human)	Lysis protein,Lysozyme
2	A	N-(adamantan-1-yl)-1-(5-hydroxypentyl)-4-methyl-5-phenyl-1H-pyrazole-3-carboxamide	non-polymer		421.6	1	Chemie (9JU)
3	A	OLEIC ACID	non-polymer		282.5	1	Chemie (OLA)
4	A	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	3	Chemie (OLC)
5	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
6	A	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	2	Chemie (EPE)
7	A	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
8	A	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
9		water	water		18.0	49	Chemie (HOH)

Sequence modifications

A: 21 - 222 (UniProt: P34972)

PDB	External Database	Details
Met 2	-	initiating methionine
Lys 3	-	expression tag
Thr 4	-	expression tag
Ile 5	-	expression tag
Ile 6	-	expression tag
Ala 7	-	expression tag
Leu 8	-	expression tag
Ser 9	-	expression tag
Tyr 10	-	expression tag
Ile 11	-	expression tag
Phe 12	-	expression tag
Cys 13	-	expression tag
Leu 14	-	expression tag
Val 15	-	expression tag
Phe 16	-	expression tag
Ala 17	-	expression tag
Gly 18	-	expression tag
Ala 19	-	expression tag
Pro 20	-	expression tag
Leu 78	Gly 78	engineered mutation
Ala 127	Thr 127	engineered mutation
Leu 153	Thr 153	engineered mutation

A: 1001 - 1160 (UniProt: D9IEF7)

PDB	External Database	Details
Thr 1053	Cys 54	engineered mutation
Ala 1096	Cys 97	engineered mutation

A: 235 - 325 (UniProt: P34972)

PDB	External Database	Details
Glu 242	Arg 242	engineered mutation
Glu 304	Gly 304	engineered mutation
Glu 326	-	expression tag
Phe 327	-	expression tag
Leu 328	-	expression tag
Glu 329	-	expression tag
Val 330	-	expression tag
Leu 331	-	expression tag
Phe 332	-	expression tag
Gln 333	-	expression tag
Gly 334	-	expression tag
Pro 335	-	expression tag
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
Asp 346	-	expression tag
Tyr 347	-	expression tag
Lys 348	-	expression tag
Asp 349	-	expression tag
Asp 350	-	expression tag
Asp 351	-	expression tag
Asp 352	-	expression tag
Lys 353	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	56195.9
Non-Polymers*	Number of molecules	15
	Total formula weight	3137.0
All*	Total formula weight	59333.0

*Water molecules are not included.