5ZSG

Crystal structure of monkey TLR7 in complex with gardiquimod

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A	Toll-like receptor 7	polymer	823	94745.6	2	UniProt (G7NS93) Pfam (PF13855) In PDB	Macaca mulatta (Rhesus macaque)
2	C, D, E, F	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
3	B, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	11	Chemie (NAG)
4	B, A	1-{4-amino-2-[(ethylamino)methyl]-1H-imidazo[4,5-c]quinolin-1-yl}-2-methylpropan-2-ol	non-polymer		313.4	2	Chemie (9K3)
5	A, B	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
6		water	water		18.0	659	Chemie (HOH)

Sequence modifications

B, A: 27 - 839 (UniProt: G7NS93)

PDB	External Database	Details
Arg 23	-	expression tag
Ser 24	-	expression tag
Pro 25	-	expression tag
Trp 26	-	expression tag
Gln 167	Asn 163	engineered mutation
Gln 389	Asn 385	engineered mutation
Leu 440	Ser 436	see sequence details
Val 441	Glu 437	see sequence details
Pro 442	Val 438	see sequence details
Arg 443	Gly 439	see sequence details
Gly 444	Phe 440	see sequence details
Ser 445	Cys 441	see sequence details
Gln 488	Asn 484	engineered mutation
Gln 799	Asn 795	engineered mutation
Glu 840	-	expression tag
Phe 841	-	expression tag
Leu 842	-	expression tag
Val 843	-	expression tag
Pro 844	-	expression tag
Arg 845	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	189491.2
Branched	Number of molecules	4
	Total formula weight	1697.6
Non-Polymers*	Number of molecules	21
	Total formula weight	3828.6
All*	Total formula weight	195017.4

*Water molecules are not included.