5ZQS

Crystal structure of beta-xylosidase mutant (E186Q/F503Y) from Bacillus pumilus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Beta-xylosidase	polymer	541	62105.5	2	UniProt (P07129) Pfam (PF04616) Pfam (PF17851) In PDB	Bacillus pumilus (Bacillus mesentericus)	1,4-beta-D-xylan xylohydrolase,Xylan 1,4-beta-xylosidase
2	C, D	beta-D-xylopyranose-(1-4)-beta-D-xylopyranose	branched		282.2	2	In PDB BIRD (PRD_900116) GlyTouCan (G87728WL)		4beta-beta-xylobiose
3	A	beta-D-xylopyranose	non-polymer		150.1	1	Chemie (XYP)
4		water	water		18.0	555	Chemie (HOH)

Sequence modifications

A, B: 1 - 535 (UniProt: P07129)

PDB	External Database	Details
Gln 186	Glu 186	engineered mutation
Tyr 503	Phe 503	engineered mutation
His 536	-	expression tag
His 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	124211.0
Branched	Number of molecules	2
	Total formula weight	564.5
Non-Polymers*	Number of molecules	1
	Total formula weight	150.1
All*	Total formula weight	124925.6

*Water molecules are not included.