5ZNO
Crystal structure of PET-degrading cutinase Cut190 S176A/S226P/R228S/ mutant in Ca(2+)-bound state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Alpha/beta hydrolase family protein | polymer | 265 | 29193.7 | 2 | UniProt (W0TJ64) Pfam (PF03403) | Saccharomonospora viridis | Cutinase |
| 2 | C, D, E, I, J... (A, B) | CALCIUM ION | non-polymer | 40.1 | 7 | Chemie (CA) | |||
| 3 | F, G, H, M, N (A, B) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 4 | O, P (A, B) | water | water | 18.0 | 789 | Chemie (HOH) |
Sequence modifications
A, B: 45 - 304 (UniProt: W0TJ64)
| PDB | External Database | Details |
|---|---|---|
| Gly 43 | - | expression tag |
| Pro 44 | - | expression tag |
| Ala 176 | Ser 176 | engineered mutation |
| Pro 226 | Ser 226 | engineered mutation |
| Ser 228 | Arg 228 | engineered mutation |
| Lys 305 | - | expression tag |
| Leu 306 | - | expression tag |
| Asn 307 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 58387.3 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 741.0 | |
| All* | Total formula weight | 59128.3 |
