5ZNO
Crystal structure of PET-degrading cutinase Cut190 S176A/S226P/R228S/ mutant in Ca(2+)-bound state
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Alpha/beta hydrolase family protein | polymer | 265 | 29193.7 | 2 | UniProt (W0TJ64) Pfam (PF03403) In PDB | Saccharomonospora viridis | Cutinase |
2 | A, B | CALCIUM ION | non-polymer | 40.1 | 7 | Chemie (CA) | |||
3 | A, B | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
4 | water | water | 18.0 | 789 | Chemie (HOH) |
Sequence modifications
A, B: 45 - 304 (UniProt: W0TJ64)
PDB | External Database | Details |
---|---|---|
Gly 43 | - | expression tag |
Pro 44 | - | expression tag |
Ala 176 | Ser 176 | engineered mutation |
Pro 226 | Ser 226 | engineered mutation |
Ser 228 | Arg 228 | engineered mutation |
Lys 305 | - | expression tag |
Leu 306 | - | expression tag |
Asn 307 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 58387.3 | |
Non-Polymers* | Number of molecules | 12 |
Total formula weight | 741.0 | |
All* | Total formula weight | 59128.3 |