5ZE2
Hairpin Complex, RAG1/2-hairpin 12RSS/23RSS complex in 5mM Mn2+ for 2 min at 4'C
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | mouse RAG1 | polymer | 627 | 71871.3 | 2 | UniProt (P15919) Pfam (PF12940) | Mus musculus (Mouse) | RAG-1 |
| 2 | B, D (B, D) | mouse RAG2 | polymer | 389 | 43225.7 | 2 | UniProt (P21784) Pfam (PF03089) | Mus musculus (Mouse) | RAG-2 |
| 3 | E (N) | HMGB1 A-B box | polymer | 163 | 18897.9 | 1 | UniProt (P63158) Pfam (PF09011) Pfam (PF00505) | Mus musculus (Mouse) | High mobility group protein 1,HMG-1 |
| 4 | F (I) | DNA (31-MER) | polymer | 31 | 9536.2 | 1 | synthetic construct | ||
| 5 | G (J) | DNA (31-MER) | polymer | 31 | 9527.1 | 1 | synthetic construct | ||
| 6 | H (G) | DNA (40-MER) | polymer | 40 | 12257.8 | 1 | synthetic construct | ||
| 7 | I (L) | DNA (30-MER) | polymer | 30 | 9138.9 | 1 | synthetic construct | ||
| 8 | J (M) | DNA (40-MER) | polymer | 40 | 12326.0 | 1 | synthetic construct | ||
| 9 | K (F) | DNA (30-MER) | polymer | 30 | 9307.0 | 1 | synthetic construct | ||
| 10 | L, R (A, C) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 11 | M, N, S, T (A, C) | MANGANESE (II) ION | non-polymer | 54.9 | 4 | Chemie (MN) | |||
| 12 | O, P, U (A, C) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 13 | Q, V (A, C) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 14 | AA, BA, W, X, Y... (L, F, A, B, C...) | water | water | 18.0 | 10 | Chemie (HOH) |
Sequence modifications
A, C: 384 - 1008 (UniProt: P15919)
B, D: 1 - 387 (UniProt: P21784)
| PDB | External Database | Details |
|---|---|---|
| Gly 382 | - | cloning artifact |
| Pro 383 | - | cloning artifact |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | cloning artifact |
| Pro 0 | - | cloning artifact |
| Val 1 | Met 1 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 311185.0 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 615.0 | |
| All* | Total formula weight | 311799.9 |
