5ZBD

Crystal structure of tryptophan oxidase (C395A mutant) from Chromobacterium violaceum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Flavin-dependent L-tryptophan oxidase VioA	polymer	451	50478.2	2	UniProt (Q9S3V1) Pfam (PF01593)	Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)	Tryptophan Oxidase
2	C, E (A, B)	TRYPTOPHAN	non-polymer		204.2	2	Chemie (TRP)
3	D, F (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
4	G, H (A, B)	water	water		18.0	760	Chemie (HOH)

Sequence modifications

A, B: 8 - 425 (UniProt: Q9S3V1)

PDB	External Database	Details
Met -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Leu 1	-	expression tag
Val 2	-	expression tag
Pro 3	-	expression tag
Arg 4	-	expression tag
Gly 5	-	expression tag
Ser 6	-	expression tag
His 7	-	expression tag
Ala 402	Cys 395	engineered mutation
Lys 426	-	expression tag
Leu 427	-	expression tag
Ala 428	-	expression tag
Ala 429	-	expression tag
Ala 430	-	expression tag
Leu 431	-	expression tag
Glu 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	100956.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1979.5
All*	Total formula weight	102935.9

*Water molecules are not included.