5ZBC

Crystal structure of Se-Met tryptophan oxidase (C395A mutant) from Chromobacterium violaceum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Flavin-dependent L-tryptophan oxidase VioA	polymer	451	51040.9	2	UniProt (Q9S3V1) Pfam (PF01593)	Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)	Tryptophan Oxidase
2	C, D (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3	E, F (A, B)	water	water		18.0	348	Chemie (HOH)

Sequence modifications

A, B: 8 - 425 (UniProt: Q9S3V1)

PDB	External Database	Details
Mse -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Leu 1	-	expression tag
Val 2	-	expression tag
Pro 3	-	expression tag
Arg 4	-	expression tag
Gly 5	-	expression tag
Ser 6	-	expression tag
His 7	-	expression tag
Ala 402	Cys 395	engineered mutation
Lys 426	-	expression tag
Leu 427	-	expression tag
Ala 428	-	expression tag
Ala 429	-	expression tag
Ala 430	-	expression tag
Leu 431	-	expression tag
Glu 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	102081.8
Non-Polymers*	Number of molecules	2
	Total formula weight	1571.1
All*	Total formula weight	103652.9

*Water molecules are not included.